Enzyme Assays
Wiley-VCH (Verlag)
978-3-527-31095-1 (ISBN)
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For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
Nowadays, the characterization of enzymes using simple assays is a key technology for finding the optimum enzymes for, among others, catalysis in enzymology and drug discovery. Thus, enzymes have become an everyday tool not only for biologists, but also for chemists, and the number of applications is now overwhelming. Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.
1985 Jean-Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zürich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.
PART I. THE CHEMISTRY OF ENZYME ASSAYS1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity2. High-throughput screening systems for assaying the enantioselectivity of enzymes3. High throughput screening methods developed for oxidoreductases4. Industrial perspective on assaysPART II. ENZYME ASSAYS AND GENETIC SELECTION5. Agar-plate based assays6. Genetic selection for biocatalysts7. Three-hybrid systems and in vivo selection8. Nucleic-acid based approaches on the screening of enzymes.PART III. ENZYME PROFILING9. Fluorescent probes for lipolytic enzymes10. Enzyme fingerprinting11. Protease profiling12. Enzyme assays on chipsINDEX
| Sprache | englisch |
|---|---|
| Maße | 170 x 240 mm |
| Gewicht | 875 g |
| Einbandart | gebunden |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Chemie ► Organische Chemie | |
| Schlagworte | Analytische Chemie • Biochemie • Biotechnologie • Enzym • Katalyse • Medizinische Chemie |
| ISBN-10 | 3-527-31095-9 / 3527310959 |
| ISBN-13 | 978-3-527-31095-1 / 9783527310951 |
| Zustand | Neuware |
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