Enzyme Assays (eBook)

High-throughput Screening, Genetic Selection and Fingerprinting

Jean-Louis Reymond (Herausgeber)

eBook Download: PDF

2006 | 1. Auflage
XVIII, 368 Seiten
Wiley-VCH (Verlag)
978-3-527-60721-1 (ISBN)

Infos

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.
For biochemists, analytical, organic and catalytic chemists, and biotechnologists.

Autor

1985 Jean-Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zürich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.

Inhalt

PART I. THE CHEMISTRY OF ENZYME ASSAYS
1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity
2. High-throughput screening systems for assaying the enantioselectivity of enzymes
3. High throughput screening methods developed for oxidoreductases
4. Industrial perspective on assays
PART II. ENZYME ASSAYS AND GENETIC SELECTION
5. Agar-plate based assays
6. Genetic selection for biocatalysts
7. Three-hybrid systems and in vivo selection
8. Nucleic-acid based approaches on the screening of enzymes.
PART III. ENZYME PROFILING
9. Fluorescent probes for lipolytic enzymes
10. Enzyme fingerprinting
11. Protease profiling
12. Enzyme assays on chips
INDEX PART I. THE CHEMISTRY OF ENZYME ASSAYS
1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity
2. High-throughput screening systems for assaying the enantioselectivity of enzymes
3. High throughput screening methods developed for oxidoreductases
4. Industrial perspective on assays
PART II. ENZYME ASSAYS AND GENETIC SELECTION
5. Agar-plate based assays
6. Genetic selection for biocatalysts
7. Three-hybrid systems and in vivo selection
8. Nucleic-acid based approaches on the screening of enzymes.
PART III. ENZYME PROFILING
9. Fluorescent probes for lipolytic enzymes
10. Enzyme fingerprinting
11. Protease profiling
12. Enzyme assays on chips
INDEX

Rezensionen

"...an excellent overview presenting a unique exposition of all important developments, techniques and concepts in this rapidly advancing field. [...] In summary, this book gives an excellent overview of current and relevant approaches where there is a need for enzyme assay development and use, and should be of interest to a broad readership. It is an indispensable source of information and inspiration for everyone working in the field and can highly be recommended."
ChemBioChem

"...the value of this volume to students or investigators lies in the ability to develop assays in one's own laboratory based on the material presented...The broad and diverse scope of this volume makes it best suited to be an acquisition by an organization's library." Clinical Chemistry

"....is a useful source of information, inspiration, and references for its readers across chemistry and biology."
International Journal of Biological Macromolecules

"Das Buch präsentiert das Thema "Enzyme Assays" in einem Konzept, wie ich es bisher noch nicht gesehen habe. Dies ist per se innovativ, im konkreten Fall aber darüber hinaus auch noch außergewöhnlich informativ."
Pharmazie in unserer Zeit

"...Insgesamt bietet dieses Buch eine beeindruckende Übersicht über den aktuellen Stand der Forschungen über Enzym-Assays, mit einer ausgewogenen Mischung aus Grundlagen, klassischen Methoden und neuen Techniken. Es ist klar gegliedert, und die Kapitel sind gut aufeinander abgestimmt, sodass kaum Überschneidungen vorkommen. Insbesondere für praxisorientierte Forscher sind die zahlreich vorhandenen Versuchsprotokolle sehr hilfreich...Überhaupt lässt sich das Buch vielfältig einsetzen: Es kann zum einen als Grundlage für eine Fortgeschrittenenvorlesung über Enzym-Assays dienen, bietet außerdem Chemikern und Biologen eine sehr gute Einführung in das Gebiet und ist nicht zuletzt eine wertvolle Inspirationsquelle für erfahrene Forscher."
Angewandte Chemie, 06/2007

Produkt-Details

• Erscheint lt. Verlag: 12.5.2006
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie ► Organische Chemie
• Themenwelt: Technik
• Schlagworte: Analytische Chemie • Biochemie • Biotechnologie • Biotechnologie i. d. Biowissenschaften • Biotechnology • Biowissenschaften • Chemie • Chemistry • Drug Discovery & Development • Enzymes & Receptors • Enzyme u. Rezeptoren • Katalyse • Life Sciences • Medizinische Chemie • Methods - Synthesis & Techniques • Organische Chemie / Methoden, Synthesen, Verfahren • Pharmaceutical & Medicinal Chemistry • Pharmazeutische u. Medizinische Chemie • Wirkstoffforschung u. -entwicklung
• ISBN-10: 3-527-60721-8 / 3527607218
• ISBN-13: 978-3-527-60721-1 / 9783527607211