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Yeast Genetics

A Manual of Methods

John F.T. Spencer, Dorothy M. Spencer, I.J. Bruce (Autoren)

Buch | Softcover

VII, 104 Seiten

1988
Springer Berlin (Verlag)
978-3-540-18805-6 (ISBN)

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This book is designed for use as a manual of methods, to be used at the bench. Topics discussed and methods described include classical genetics as related to yeasts, such as mating, sporulation, isolation of hybrids, microdissection of asci or the isolation of single-spore clones, mapping of genes and the construction of new strains by protoplast fusion. There is a large section dealing with mutations in general, and with methods of isolating a number of important classes of mutants in particular. A second part deals with basic techniques in separation of chromosomes by electrophoresis, such as OFAGE, FIGE, and CHEF, and gives detailed protocols for the first two. This section further includes methods for isolation of high molecular weight DNA from yeast, isolation of RNA, and techniques for transformation of yeasts.

Contents: Introduction.- "Classical" Yeast Genetics: Mating. Sporulation. Tetrad Analysis. Enzymes for Digestion of Yeast Ascus Walls. Ancillary Methods for Handling Clones Obtained by Ascus Dissection. Mutagenesis. Mapping and Fusion. Protoplast Formation and Fusion.- Methods Using Direct Manipulation of DNA and RNA: Separation of Large DNA Molecules, Greater than 25 Kbp, by Gel Electrophoresis. Isolation of Pure High-Molecular-Weight DNA from the Yeast Saccharomyces cerevisiae. Transformation of Yeast: Saccharomyces cerevisiae. Plasmid Isolation from Yeast. Rapid Isolation of DNA from Yeast. RNA Isolation.- Subject Index.

Erscheint lt. Verlag	23.12.1988
Reihe/Serie	Manuals for the Bench
Zusatzinfo	VII, 104 p. 4 illus.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	225 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	chromosome • DNA • Enzyme • enzymes • Genetics • Genetik • Genetik / Erblehre • Mutagenesis • Mutation • Protoplast • RNA • yeast
ISBN-10	3-540-18805-3 / 3540188053
ISBN-13	978-3-540-18805-6 / 9783540188056
Zustand	Neuware