Sedimentation Velocity Analytical Ultracentrifugation
CRC Press (Verlag)
978-0-367-87828-3 (ISBN)
The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems.
Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx
Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997. He is currently a Earl Stadtman Tenure-Track Investigator and Chief of the Dynamics of Macromoleular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics at the National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health.
Basic Analysis Principles
Sedimentation of Discrete Non-Interacting Particles
Properties of Sedimentation Coefficient Distributions
Distributions Of Non-Diffusing Particles
Distributions of Diffusing Particles
Sedimentation Coefficient Distributions from Boundary Derivatives and Extrapolations
Multi-Component Distributions
Practical Analysis of Non-Interacting Systems
Appendix A: Numerical Solutions of the Lamm Equation
Appendix B: Calculating Distributions
Erscheinungsdatum | 23.12.2019 |
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Verlagsort | London |
Sprache | englisch |
Maße | 178 x 254 mm |
Gewicht | 453 g |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik | |
Technik | |
ISBN-10 | 0-367-87828-3 / 0367878283 |
ISBN-13 | 978-0-367-87828-3 / 9780367878283 |
Zustand | Neuware |
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