Enzyme-Mediated Ligation Methods
Humana Press Inc. (Verlag)
978-1-4939-9545-5 (ISBN)
Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions.
Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates.- Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL).- Cyclizing Disulfide-Rich Peptides using Sortase A.- Chemo-Enzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1.- Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE).- Butelase 1-Mediated Ligation of Peptides and Proteins.- Trypsiligase-Catalyzed Peptide and Protein Ligation.- Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase.- Tailoring Activity and Selectivity of Microbial Transglutaminase.- SpyLigase-Catalyzed Modification of Antibodies.- Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases.- In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis.- Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation.- Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization.- BioID as a Tool for Protein Proximity Labeling in Living Cells.- N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells.- Tubulin Tyrosine Ligase-Mediated Modification of Proteins.- Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine.
Erscheinungsdatum | 24.06.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 2012 |
Zusatzinfo | 59 Illustrations, color; 35 Illustrations, black and white; XI, 371 p. 94 illus., 59 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | biotherapeutics • Cyclization • Glutathione-S-Transferase • Peptide Coupling • phosphopantheinyl transferase |
ISBN-10 | 1-4939-9545-6 / 1493995456 |
ISBN-13 | 978-1-4939-9545-5 / 9781493995455 |
Zustand | Neuware |
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