Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres

Yusuke Kawamoto (Autor)

Buch | Hardcover

122 Seiten

2019 | 1st ed. 2019
Springer Verlag, Singapore
978-981-13-6911-7 (ISBN)

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This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites c

1. General Introduction Sequence-specific DNA Binding Pyrrole–Imidazole Polyamides and Their Applications.- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole–Imidazole Polyamide Probes Targeting Human Telomeres.- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences.- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole−Imidazole Polyamide Probes.

Erscheinungsdatum	22.03.2019
Reihe/Serie	Springer Theses
Zusatzinfo	52 Illustrations, color; 10 Illustrations, black and white; XII, 122 p. 62 illus., 52 illus. in color.
Verlagsort	Singapore
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Chemie ► Organische Chemie
	Technik ► Maschinenbau
Schlagworte	Minor groove binder • Pyrrole-imidazole Polyamide • solid-phase peptide synthesis • Telomere • Visualization of Telomeres
ISBN-10	981-13-6911-9 / 9811369119
ISBN-13	978-981-13-6911-7 / 9789811369117
Zustand	Neuware