Techniques in Glycobiology
Crc Press Inc (Verlag)
978-0-8247-9822-2 (ISBN)
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R. Reid Townsend, Arland T. Hotchkiss
Mass spectrometry and NMR: new frontiers in nuclear magnetic resonance spectroscopy - use of the Nano.NMR probe for the analysis of microgram quantities of complex carbohydrates; analysis of anionic glycoconjugates by delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Glycolipids and the new physical methods: microscale analysis of glycosphingolipids by thin-layer chromatography blotting; high-performance liquid chromatography of gangliosides; a sensitive cell suspension enzyme-linked immunosorbent assay measuring the density of cell surface ganglioside and carbohydrate antigens. Protein 0-glycosylation: effects of 0-glycosylation on protein structure - the role and characterization of heavily 0-glycosylated mucin domains; predicting mucin-type 0-glycosylation sites; experimental approaches to studying 0-glycosylation in vivo. Labelling oligosaccharides with chromophore and fluorophores: highly sensitive pre-column derivation procedures for quantitative determination of monosaccharides, sialic acids, and amino sugar alcohols of glycoproteins by reversed phase high-performance liquid chromatography; glycan labelling with the fluorophores 2-aminobenzamide and anthranilic acid. Strategies for oligosaccharides separations and sequencing: discovery and uses of novel glucosidases: resolution of oligosaccharides in capillary electrophoresis; a comparison of oligosaccharides profiling methods. Plant glycobiology: recent developments in starch structure analysis using high-performance anion-exchange chromatography with pulsed amperometric detection; red seaweed galactants - methodology for the structural determination of corallinan, a different agaroid. (Part contents)>
| Erscheint lt. Verlag | 25.6.1997 |
|---|---|
| Verlagsort | Bosa Roca |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| ISBN-10 | 0-8247-9822-8 / 0824798228 |
| ISBN-13 | 978-0-8247-9822-2 / 9780824798222 |
| Zustand | Neuware |
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