Protein Folding Kinetics
Springer Berlin (Verlag)
978-3-540-27277-9 (ISBN)
Protein Folding Kinetics - Biophysical Methods (2nd Edition) gives a deep insight into the principles and concepts of the kinetic and structural resolution of fast chemical and biophysical reactions of proteins with emphasis on protein-folding reactions. The study of fast protein-folding reactions and the understanding of the folding paradox have significantly advanced due to the recent development of new biophysical methods which allow not only kinetic resolution in the sub-millisecond time scale but also structural resolution with unprecedented precision. Pathways and structures of early and late folding events and the transition state structures of fast- and ultrafast-folding proteins can now be studied in far more detail. Important techniques include biophysical, chemical, molecular biological and mathematical methods, in particular protein engineering, Phi-value analysis, time-resolved circular dichroism, optical triggers and pulsed infrared LASER methods, pressure and temperature jump, ultrafast mixing, stopped flow and quenched flow, dielectric relaxation and electric-field-jump, acoustic relaxation, fluorescence- and isotope-labeling, H/D exchange methods, NMR line broadening and stopped-flow NMR, transition state theory, solutions of rate equations, and evolutionary computer programming. Protein Folding Kinetics - Biophysical Methods is written for students and researchers in biochemistry, biophysics, and related fields.
Special features in the second edition:
-Includes detailed information and 12 color figures on the high resolution of folding transition states.
-Discusses structural determinants of the rate of protein folding on a timescale from microseconds to seconds.
-Provides information on self-evolving computer programs for protein-folding simulations and protein-structure predictions.
Structures of proteins.- Physical interactions that determine the properties of proteins.- Calculation of the kinetic rate constants.- High kinetic resolution of protein folding events.- Kinetic methods for slow reactions.- Resolution of protein structures in solution.- High structural resolution of transient protein conformations.- Experimental problems of the kinetic and structural resolution of reactions that involve proteins.- The folding pathway of a protein (barstar) at the resolution of individual residues from microseconds to seconds.- Highly resolved folding pathways and mechanisms of six proteins.- Structural determinants of the rate of protein folding.- Evolutionary computer programming of protein structure and folding.- Conclusions.
| Erscheint lt. Verlag | 24.8.2005 |
|---|---|
| Zusatzinfo | XVI, 222 p. 170 illus., 12 illus. in color. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 554 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | biochemistry • Biophysics • Biotechnologie • Biotechnology • Enzyme • enzymes • fluorescence • Kinetics • Kinetik • Methoden • Methods • Protein • Proteine • Protein / Eiweiß • proteins • Temperature |
| ISBN-10 | 3-540-27277-1 / 3540272771 |
| ISBN-13 | 978-3-540-27277-9 / 9783540272779 |
| Zustand | Neuware |
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