Practical Protein Chromatography

Andrew Kenney, Susan Fowell (Autoren)

Buch | Hardcover

327 Seiten

1992
Humana Press Inc. (Verlag)
978-0-89603-213-2 (ISBN)

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Brings the forefront methods of protein separation and purification directly to your laboratory benchtop. This book treats not only analytical separations, but also the small- and large-scale preparative techniques that are applicable throughout biological and biomedical research. It is suitable for those who are doing protein chromatography.

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Thiophilic Adsorption Chromatography.- Immobilized Metal Ion Affinity Chromatography.- Histidine Ligand Affinity Chromatography.- Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes.- Calcium-Dependent Hydrophobic Interaction Chromatography.- Lectin Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- High-Performance Affinity Chromatography for Protein Separation and Purification.- Immunoafflnity Chromatography.- Selection of Antibodies for Immunoaffinity Chromatography.- Some Alternative Coupling Chemistries for Affinity Chromatography.- Exploiting Weak Affinities.- Biospecific Affinity Elution.- Size-Exclusion High-Performance Liquid Chromatography of Proteins.- Chromatofocusing.- Ion-Exchange Chromatography of Proteins.- Displacement Chromatography of Proteins.- Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography.- Making Bacterial Extracts Suitable for Chromatography.- Determination of Purity and Yield.

...an indispensable, hands-on guide... -Unlisted Drugs

Erscheint lt. Verlag	9.4.1992
Reihe/Serie	Methods in Molecular Biology ; 11
Zusatzinfo	7 Illustrations, black and white; X, 327 p. 7 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Chemie ► Analytische Chemie
ISBN-10	0-89603-213-2 / 0896032132
ISBN-13	978-0-89603-213-2 / 9780896032132
Zustand	Neuware