Reactive Oxygen Species in Plants

Methods and Protocols

Buch | Softcover

298 Seiten

2023 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2471-5 (ISBN)

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This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.

lt;p>Part I: Strategies to Induce ROS Production

1. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen, and Yi Han

2. Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress

Takanori Maruta and Takahiro Ishikawa

3. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang, Wei Xuan, and Yi Han

4. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

Alexey Shapiguzov and Jaakko Kangasjärvi

Part II: Methods to Visualize ROS and to Detect Changes in Redox Homeostasis

5. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer

6. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

Graham Noctor and Amna Mhamdi

7. Measurement of NAD(P)H and NADPH-Generating Enzymes

Amna Mhamdi, Frank Van Breusegem, and Graham Noctor

8. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, and Kazuyuki Kuchitsu

Part III: Small-Scale Targeted Analysis of ROS Accumulation during Stress and Effects on Plant Physiology

9. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

Jagna Chmielowska-Bak, Karolina Izbianska-Jankowska, and Joanna Deckert

10. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

Corentin Moreau and Emmanuelle Issakidis-Bourguet

11. In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation

Zeya Chen and Jingjing Huang

12. Methods to Analyze the Redox Reactivity of Plant Proteins

Thualfeqar Al-Mohanna, George V. Popescu, and Sorina C. Popescu

13. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

Brigitte Ksas and Michel Havaux

14. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions

Attila L. Ádám, György Kátay, András Künstler, and Lóránt Király

15. Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells

Jun'ichi Mano, Md. Sanaullah Biswas, Koichi Sugimoto, and Yoshiyuki Murata

16. Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

Caroline Lelarge-Trouverie, Amna Mhamdi, Florence Guérard, and Graham Noctor

Part IV: Systems Biology Approaches to Understand ROS Functions

17. Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM)

Jesús Pascual and Saijaliisa Kangasjärvi

18. Quantitative Analysis of Post-Translational Modifications of Plant Histones

Hana Kucharíková, Zuzana Plsková, Zbynek Zdráhal, Miloslava Fojtová, Pavel Kerchev, and Gabriela Lochmanová

19. Characterization of RBPome in Oxidative Stress Conditions

Zhicheng Zhang, Evy Timmerman, Francis Impens, and Frank Van Breusegem

20. Analysis of ROS-Triggered Changes in the Transcriptome

Patrick Willems

Erscheinungsdatum	08.06.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	36 Illustrations, color; 17 Illustrations, black and white; XIV, 298 p. 53 illus., 36 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Arabidopsis thaliana • Effects of Climate Change • Plant metabolism • Redox homeostasis • ROS production • stress signaling • Systems biology approaches
ISBN-10	1-0716-2471-7 / 1071624717
ISBN-13	978-1-0716-2471-5 / 9781071624715
Zustand	Neuware