Difference Gel Electrophoresis
Springer-Verlag New York Inc.
978-1-0716-2830-0 (ISBN)
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Two-Dimensional Gel Electrophoresis and 2D-DIGE.- Top-down Proteomics and Comparative 2D-DIGE Analysis.- DIGE Analysis Software and Protein Identification Approaches.- Native DIGE for Quantitative and Functional Analysis of Protein Interactomes.- Comparative Two-dimensional Fluorescence Gel Electrophoresis.- Identification of Ubiquitination associated Proteins using 2D-DIGE.- DIGE-based Phosphoproteomic Analysis.- DIGE-based Biomarker Discovery in Blood Cancers.- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue.- DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples.- Comparative 3-sample 2D-DIGE Analysis of Skeletal Muscles.- Proteomic identification of saliva proteins as non-invasive diagnostic biomarkers.- Analysis of the saliva proteome using 2D-DIGE.- DIGE Analysis of Clinical Specimens.- DIGE Analysis of Animal Tissues.- Rapid 2D-DIGE Proteomic Analysis of Mouse Liver within One Week.- 2D-DIGE analysis of liver disease in mice.- 2-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis.- 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens.- Identification of Subproteomic Markers for Skeletal Muscle Profiling.- DIGE Analysis of Fish Tissues.- Sample preparation and protein determination for 2D-DIGE proteomics.- Protein Digestion for 2D-DIGE Analysis.- Subcellular Fractionation for DIGE-based Proteomics.- DIGE Analysis of Immunodepleted Plasma.- Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue.- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays.- Enzyme Assay Methods to Validate DIGE Proteomics Data.- Immunoblot Analysis of DIGE-based Proteomics.- Verification of Protein Changes determined by 2D-DIGE based Proteomics using Immunofluorescence Microscopy.- Histological and Histochemical Microscopy used to verify 2D-DIGE Pathoproteomics.
Erscheinungsdatum | 19.11.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2596 |
Zusatzinfo | 74 Illustrations, color; 8 Illustrations, black and white; XII, 485 p. 82 illus., 74 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Chemie | |
Schlagworte | 2D-DIGE • DIGE-Based • Ffpe • Glycoproteomic Analysis • Post-translational modifications |
ISBN-10 | 1-0716-2830-5 / 1071628305 |
ISBN-13 | 978-1-0716-2830-0 / 9781071628300 |
Zustand | Neuware |
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