Histochemistry of Single Molecules

Methods and Protocols

Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta (Herausgeber)

Buch | Hardcover

357 Seiten

2022 | 2nd ed. 2023
Springer-Verlag New York Inc.
978-1-0716-2674-0 (ISBN)

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This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy.

Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes.- Autofluorescence Label-Free Imaging of Liver Reticular Structure.- Lysosome Imaging Based on Fluorescent Carbon Dots.- Oxidative and Nitrosative Stress Detection in Human Sperm using Fluorescent Probes.- Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis.- Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions.- Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans.- Combined Lectin- and Immuno-Histochemistry (CLIH) for Fluorescence Microscopy.- Immunofluorescence Labelling of Skeletal Muscle in Development, Regeneration, and Disease.- Immunohistochemical Detection of the Autophagy Markers LC3 and P62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissues.- Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin Fixed and Paraffin Embedded Tissues.- Immunogold Labelling of Milk Proteins at Transmission Electron Microscopy.- Rediscover Potassium Permanganate as a Stain for Basic Proteins on Ultrathin Sections at Transmission Electron Microscopy.- Tissue Fixation and Processing for the Histological Identification of Lipids.- Staining Methods for Normal and Regenerative Myelin in the Nervous System.- Nile Red and BODIPY Staining Of Lipid Droplets in Mouse Oocytes and Embryos.- Chromatin Dispersion Test to Assess DNA Damage in Cervical Epithelial Cells.- Uranyl-free staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy.- Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapours.- Localising Molecules in Plant Cell Walls using Fluorescence Microscopy.- Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro.- Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls.- Staining Starch with Iodine Solution.- Histochemical Analysis of Plant Secretory Structures.- Alcian Blue Staining to Visualize Intracellular Hyaluronic-Acid-Based Nanoparticles.- Prussian Blue Staining to Visualize Iron Oxide Nanoparticles.- Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy.- Fluorescent Labelling of Lignin Nanocapsules with Fluorol Yellow 088.

Erscheinungsdatum	17.10.2022
Reihe/Serie	Methods in Molecular Biology ; 2566
Zusatzinfo	71 Illustrations, color; 11 Illustrations, black and white; XV, 357 p. 82 illus., 71 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Schlagworte	autofluorescence • epithelial-mesenchymal transition • extracellular matrix • lectin histochemistry • Spermatozoa
ISBN-10	1-0716-2674-4 / 1071626744
ISBN-13	978-1-0716-2674-0 / 9781071626740
Zustand	Neuware