PDZ Mediated Interactions

Methods and Protocols

Buch | Softcover

292 Seiten

2021 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1168-5 (ISBN)

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This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field.

Identification of PDZ Interactions by Yeast Two-Hybrid.- Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis.- Identification of PDZ Interactions by Proteomic Peptide Phage Display.- A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay.- Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore.- PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations.- Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module.- A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics.- Unveiling the Folding Mechanism of PDZ Domains.- Development of Peptide-Based PDZ Domain Inhibitors.- Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs.- Chemical Synthesis of PDZ Domains.- Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains.- Computational Design of PDZ:peptide Binding.- Mechanoregulation of PDZ Proteins, An Emerging Function.- Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains.

Erscheinungsdatum	26.05.2022
Reihe/Serie	Methods in Molecular Biology ; 2256
Zusatzinfo	50 Illustrations, color; 50 Illustrations, black and white; XI, 292 p. 100 illus., 50 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	PDZ domains • peptide phage display • protein-lipid interactions • super-binder peptides • two-hybrids in the yeast
ISBN-10	1-0716-1168-2 / 1071611682
ISBN-13	978-1-0716-1168-5 / 9781071611685
Zustand	Neuware