Quantitative Methods in Proteomics
Springer-Verlag New York Inc.
978-1-0716-1026-8 (ISBN)
Authoritative and cutting-edge, Quantitative Methods in Proteomics, Second Edition aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics.
Important Issues in Planning a Proteomics Experiment: Statistical Considerations of Quantitative Proteomic Data.- Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis.- Protein Quantification using the “Rapid Western Blot” Approach.- The Whereabouts of 2D Gels in Quantitative Proteomics.- Proteome Analysis with Classical 2D‑PAGE.- Silver Staining of 2D Electrophoresis Gels.- Differential Proteome Analysis using 2D-DIGE.- Quantitative Mass Spectrometry-Based Proteomics: An Overview.- Tandem Mass Tags for Comparative and Discovery Proteomics.- An Approach for Triplex-IPTL.- Targeted Protein Quantification Using Parallel Reaction Monitoring (PRM).- Quantitative Approach using Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-ToF) Mass Spectrometry.- Application of SILAC Labeling in Phosphoproteomics Analysis.- Relative Quantification Of Phosphorylated and Glycosylated Peptides from the Same Sample using Isobaric Chemical Labelling with a Two-step Enrichment Strategy.- High Throughput Profiling of Proteome and Posttranslational Modifications by 16-plex TMT Labeling and Mass Spectrometry.- Quantification and Identification of Post-Translational Modifications using Modern Proteomics Approaches.- Affinity-enrichment Chemoproteomics for Target Deconvolution and Selectivity Profiling.- 2nSILAC for Quantitative Proteomics of Prototrophic Baker's Yeast.- Metabolic Labeling of Clostridioides difficile Proteins.- Application of Label-Free Proteomics for Quantitative Analysis of Urothelial Carcinoma and Cystitis Tissue.- Quantitative MS Workflow for a High-Quality Secretome Analysis by a Quantitative Secretome-proteome Comparison.- Establishing a Custom-Fit Data Independent Acquisition Method for Label Free Proteomics.- Label-free Proteomics Of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry.- DIA-MSEto Study Microglial Function in Schizophrenia.- Detailed Method for Performing the ExSTA Approach in Quantitative Bottom-up Plasma Proteomics.- Quantitative Cross-Linking of Proteins and Protein Complexes.- Missing Value Monitoring to Address Missing Values in Quantitative Proteomics.- Quantitative Proteome Data Analysis of Tandem Mass Tags Labeled Samples.- Mining Protein Expression Databases Using Network Meta-Analysis.- A Tutorial for Variance-sensitive Clustering And The Quantitative Analysis Of Protein Complexes.- Automated Workflow for Peptide-level Quantitation from DIA/ SWATH-MS Data.
| Erscheinungsdatum | 11.05.2022 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2228 |
| Zusatzinfo | 65 Illustrations, color; 20 Illustrations, black and white; XV, 483 p. 85 illus., 65 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | 2D-DIGE • iTRAQ • Plasma analysis, • Proteomics • Quantitative Secretomics • Westernblotting |
| ISBN-10 | 1-0716-1026-0 / 1071610260 |
| ISBN-13 | 978-1-0716-1026-8 / 9781071610268 |
| Zustand | Neuware |
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