DNA and RNA Isolation Techniques for Non-Experts

Akash Gautam (Autor)

Buch | Hardcover

XVI, 203 Seiten

2022 | 1st ed. 2022
Springer International Publishing (Verlag)
978-3-030-94229-8 (ISBN)

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This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).

1-Basic Idea About Nucleic Acids For Non-Experts.- 2-General Steps During Isolation Of DNA And RNA.- 3-Phenol-Chloroform DNA Isolation Method.- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method.- 5-Spin Column-Based Isolation Of Nucleic Acid.- 6-Isolation Of Plasmid DNA By Alkaline Lysis.- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment.- 8-Lithium Chloride Based Isolation Of DNA And RNA.- 9-Isolation Of Bacteriophage DNA By Peg Method.- 10-DNA Isolation By Chelex Method.- 11-DNA Isolation By Lysozyme And Proteinase K.- 12-Salting-Out Method For Blood Samples.- 13-CTAB Or SDA Based Isolation Of Plant's DNA.- 14-DNA Extraction By Spooling Method.- 15-Magnetic Bead-Based Nucleic Acid Isolation.- 16-Density Gradient-Based Nucleic Acid Isolation.- 17-DNA Extraction From Agarose Gel Though Paper-Strips.- 18-Transformation Or Genetic Modification Of Cells/Organisms.- 19-Gene Cloning And Vectors.- 20-Polymerase Chain Reaction (PCR).- 21-Southern And Northern Blotting.- 22-Genome Mapping.- 23-Applications Of DNA Sequencing Technologies For Current Research.

Erscheinungsdatum	31.03.2022
Reihe/Serie	Techniques in Life Science and Biomedicine for the Non-Expert
Zusatzinfo	XVI, 203 p. 58 illus., 48 illus. in color.
Verlagsort	Cham
Sprache	englisch
Maße	155 x 235 mm
Gewicht	487 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Bacteriophage • Chelex method • Forensic Science • Genetic Modification • Genetics • genotyping and polymorphism • immunology • Molecular Biology • mt DNA • Nucleic acid • phenol-chloroform • proteinase-K • restriction site mapping • sequencing • southern blotting
ISBN-10	3-030-94229-5 / 3030942295
ISBN-13	978-3-030-94229-8 / 9783030942298
Zustand	Neuware