Glycan Microarrays

Methods and Protocols

Michelle Kilcoyne, Jared Q. Gerlach (Herausgeber)

Buch | Hardcover

253 Seiten

2021 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2147-9 (ISBN)

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This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins, various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.

Neoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins.- Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system.- Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies.- Nanocube-based fluidic glycan array.- General strategies for glycan microarray data processing and analysis.- Calculating half maximal inhibitory concentration (IC50) values from glycomics microarray data using GraphPad Prism.- Synthetic plant glycan microarrays as tools for plant biology.- Mucin purification and printing natural mucin microarrays.- Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins.- Tissue glycome mapping: Lectin microarray-based differential glycomic analysis of formalin-fixed paraffin-embedded tissue sections.- Detection of autoantibodies using combinatorial glycolipid microarrays.- Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development.- Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system.- Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation.- Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays.

Erscheinungsdatum	04.01.2022
Reihe/Serie	Methods in Molecular Biology ; 2460
Zusatzinfo	65 Illustrations, color; 7 Illustrations, black and white; XI, 253 p. 72 illus., 65 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Schlagworte	Cancer glycobiology • glycoprotein • microarray scanning • Mirage • neoglycoconjugate printing
ISBN-10	1-0716-2147-5 / 1071621475
ISBN-13	978-1-0716-2147-9 / 9781071621479
Zustand	Neuware