Dynamin Superfamily GTPases
Springer-Verlag New York Inc.
978-1-0716-0678-0 (ISBN)
Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.
Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae.- Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems.- A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli.- Affinity Purification and Functional Characterization of Dynamin-related Protein 1.- Purification and Characterization of MxB.- Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding.- Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions.- Nucleotide-dependent Dimerization and Conformational Switching of Atlastin.- Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System.- Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy.- Electrophysiological MethodsFor Detection Of Membrane Leakage and Hemifission by Dynamin 1.- Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.- Cellular Assays for Measuring Dynamin Activity in Muscle Cells.- Measuring Drp1 Activity in Mitochondrial Fission in vivo.- Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells.- Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells.
Erscheinungsdatum | 21.06.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2159 |
Zusatzinfo | 33 Illustrations, color; 20 Illustrations, black and white; X, 221 p. 53 illus., 33 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Chemie | |
Schlagworte | ATPases • Dynamin-1 • Mitofusins • NMR • Post-translational modifications |
ISBN-10 | 1-0716-0678-6 / 1071606786 |
ISBN-13 | 978-1-0716-0678-0 / 9781071606780 |
Zustand | Neuware |
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