Amino Acid Analysis

Methods and Protocols

Buch | Softcover

455 Seiten

2020 | 2nd ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9641-4 (ISBN)

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Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method; another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Ultra-Sensitive Quantification of Recombinant Proteins using AAA-MS.- Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry.- Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry.- Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content.- Analysis of Hydroxyproline in Collagen Hydrolysates.- Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope Resolved Metabolomics using Ultrahigh Resolution Mass Spectrometry Coupled with Direct Infusion.- Preparative HPLC Separation of Underivatized Amino Acids for Isotopic Analysis.- Quantitative Analysis of Underivatized Amino Acids by Liquid Chromatography-Tandem Mass Spectrometry.- Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry.- The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and other Amino Acids.- Analysis of Tryptophan and Its Metabolites by High-Performance Liquid Chromatography.- Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis.- Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS.- Amino Acid Analysis in Physiological Samples by GC-MS with Propylchloroformate Derivatization and iTRAQ-LC-MS/MS.- Combination of an AccQ·Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids.- Methods for Absolute Quantification of Human Plasma Free Amino Acids by High–Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry using Precolumn Derivatization.- Analysis and Enantioseparation of Amino Acids by Liquid Chromatography.- Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas Chromatography.- High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis without Derivatization.- A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids.- Chiral Analysis of Non-Protein Amino Acids by Capillary Electrophoresis.- Amino Acid Analysis via LC–MS Method after Derivatization with Quaternary Phosphonium.- Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry.- Capillary Electrophoresis of Free Amino Acids in Physiological Fluids without Derivatization Employing Direct or Indirect Absorbance Detection.- New Advances in Amino Acid Profiling in Biological Samples by Capillary Electrophoresis-Mass Spectrometry.- Enhanced Separation Capability of Sequential Injection Chromatography for Fluorimetric Determination of Intracellular Dissolved Free Amino Acids in Marine Microalgae.- Sensitive and Selective Amino Acid Profiling of Minute Tissue Amounts by HPLC/Electrospray Negative Tandem Mass Spectrometry using 9-Fluorenylmethoxycarbonly (Fmoc-Cl) Derivatization.- Wheat Gluten Amino Acid Analysis by High-Performance Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection.- Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry.- Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS (Improved Method).- Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with On-Line Multiple Detection.- Non-Marking Collection of Amino Acids from Fingerprints using Hydrogels.- Amino Acid Profiling from Fingerprints using Amide Stationary Phase UPLC-MS.

Erscheinungsdatum	21.08.2020
Reihe/Serie	Methods in Molecular Biology ; 2030
Zusatzinfo	45 Illustrations, color; 59 Illustrations, black and white; XVII, 455 p. 104 illus., 45 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Chemie ► Analytische Chemie
Schlagworte	Liquid chromatography • Mass Spectrometry • Metabolic Phenotypes • Phenylthiocarbamyl Amino Acid Adducts • RP-HPLC
ISBN-10	1-4939-9641-X / 149399641X
ISBN-13	978-1-4939-9641-4 / 9781493996414
Zustand	Neuware