High-Throughput Protein Production and Purification
Humana Press Inc. (Verlag)
978-1-4939-9626-1 (ISBN)
Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
Part 1: General procedures for high-throughput protein production.- Overview of high-throughput cloning methods for the post-genomic era.- Overview of a high-throughput pipeline for streamlining the production of recombinant proteins.- Semi-automated small-scale purification method for high-throughput expression analysis of recombinant proteins.- High-Throughput Protein Production in Yeast.- A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells.- A High-throughput Automated Protein Folding System.- Part 2: High-throughput Protocols adapted to the production of specific protein families.- High-throughput production of oxidized animal toxins in Escherichia coli.- High-Throughput purification of protein kinases in Escherichia coli and insect cells.- Parallelized micro-scale expression of soluble scFv.- High throughput production of influenza virus-like particle (VLP) array by using VLP-factoryTM, a MultiBac baculoviral genome customized for enveloped VLPexpression.- High-throughput Protein Production of membrane proteins in Saccharomyces cerevisiae.- High-throughput E. coli cell-free expression: From PCR product design to functional validation of GPCR.- High-Throughput Site-Directed Mutagenesis.- Part 3: High-throughput protein production combined with High-throughput micro-characterization for protein stability, solubility, quality and interactions.- Hot CoFi blot – A High-throughput colony-based screen for identifying more thermally stable protein variants.- High-throughput isolation of soluble protein domains using the bipartite split-GFP system.- High-throughput Analytical Light Scattering for Protein Quality Control and Characterization.- High-throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography.- A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production.- Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions.- High-throughput protein-protein interaction assays using tripartite split-GFP complementation.- High-throughput production of a new library of human single and tandem PDZ domains allows quantitative PDZ-peptide interaction screening through high throughput holdup assay.- High throughput protein analysis using Negative Stain Electron Microscopy and 2D classification.- High-throughput protein production combined with High throughput SELEX identifies an extensive atlas of Ciona robusta transcription factor DNA-binding specificities.- High-throughput Micro-characterization of RNA-protein interactions.
| Erscheinungsdatum | 19.07.2020 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2025 |
| Zusatzinfo | 106 Illustrations, color; 39 Illustrations, black and white; XVI, 537 p. 145 illus., 106 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Micro-characterization • Protein Expression • Soluble yield • structural genomics • Up-scaling production |
| ISBN-10 | 1-4939-9626-6 / 1493996266 |
| ISBN-13 | 978-1-4939-9626-1 / 9781493996261 |
| Zustand | Neuware |
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