Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Buch | Hardcover

430 Seiten

2020
Academic Press Inc (Verlag)
978-0-12-821151-9 (ISBN)

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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.

David Chenoweth is at University of Pennsylvania, PA, USA

Fluorogenic detection of protein aggregates in live cells using the AggTag method
Kwan Ho Jung and Xin Zhang

Synthesis and application of a ratiometric probe for hydrogen peroxide
Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

Protein labeling for FRET with methoxycoumarin and acridonylalanine
Chloe M. Jones, Yarra Venkatesh and E. James Petersson

Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays
Paul T. Ludford III and Yitzhak Tor

Method to discriminate amyloids using fluorescent probes
Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives
Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer
John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles
Nanxi Wang and Lei Wang

4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy
Arusha Acharyya, Ismail A. Ahmed and Feng Gai

Dissecting contributions of catalytic and reader domains in regulation of histone demethylation
Nektaria Petronikolou, James E. Longbotham and Danica Galonic Fujimori

Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization
Qing Zhang, Yue Zhang and Xuechen Li

Chemical synthesis of di-ubiquitin modified histones for further biochemical studies
Jia-Bin Li, Jun Liang and Changlin Tian

Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies
Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

FlICk (fluorescent isoindole crosslinking) for peptide stapling
Mihajlo Todorovic and David M. Perrin

Selective protein N-terminal labeling with N-hydroxysuccinimide esters
Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

Covalent live-cell labeling of proteins using a photoreactive fluorogen
Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

Photoconvertible diazaxanthilidene dyes for live cell imaging
Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

Wei Sheng, James H. Geiger and Babak Borhan

Erscheinungsdatum	08.06.2020
Reihe/Serie	Methods in Enzymology
Verlagsort	San Diego
Sprache	englisch
Maße	152 x 229 mm
Gewicht	770 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-12-821151-2 / 0128211512
ISBN-13	978-0-12-821151-9 / 9780128211519
Zustand	Neuware