Peptide Nucleic Acids

Methods and Protocols

Buch | Hardcover

308 Seiten

2020 | 3rd ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0242-3 (ISBN)

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This third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book. Chapters focus on in vivo properties and behavior and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Fmoc-based Assembly of PNA Oligomers: Manual and Microwave-assisted Automated Synthesis.- A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers.- Synthesis of Pyrrolidinyl PNA and Its Site-specific Labeling at Internal Positions by Click Chemistry.- Synthesis of PNA-peptide Conjugates as Functional SNARE Protein Mimetics.- Lipid-modified Peptide Nucleic Acids – Synthesis and Application to Programmable Liposome Fusion.- Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimido-thioether Linkage.- PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries.- Transcription Inhibition by PNA-induced R-loops.- Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA.- In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA).- Reactive Quantum Dot-based FRET Systems for Target-catalyzed Detection of RNA.- Peptide Nucleic Acids for MicroRNAs Targeting.- Detection of Microorganismsby Fluorescence in situ Hybridization using Peptide Nucleic Acid.- PNA Antisense Targeting in Bacteria. Determination of Antibacterial Activity (MIC) of PNA-peptide Conjugates.- In vivo Administration of Splice Switching PNAs using the mdx Mouse as a Model System.- Near Infrared in vivo Whole Body Fluorescence Imaging of PNA.- Poly(lactic-co-glycolic acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo.- Preparation of Conjugates for Affibody-based PNA-mediated Pretargeting.

Erscheinungsdatum	28.02.2020
Reihe/Serie	Methods in Molecular Biology ; 2105
Zusatzinfo	41 Illustrations, color; 80 Illustrations, black and white; XI, 308 p. 121 illus., 41 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-10	1-0716-0242-X / 107160242X
ISBN-13	978-1-0716-0242-3 / 9781071602423
Zustand	Neuware