The Ubiquitin Proteasome System
Humana Press Inc. (Verlag)
978-1-4939-9371-0 (ISBN)
Thorough and authoritative, The UbiquitinProteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.
Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms.- Single Turnover RING/U-Box E3-Mediated Lysine Discharge Assays.- Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins.- Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for their Ubiquitin Ligase Partners.- Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity.- Enzymatic Assembly of Ubiquitin Chains.- Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions.- Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries.- Integrated Proteogenomic Approach for Identifying Degradation Motifs in Eukaryotic Cells.- A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting.- E. coli Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins.- Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates during E3-Mediated Ubiquitin-Like Protein Ligation.- Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway.- Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes.- Recombinant Expression, Unnatural Amino-Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes.- Native Gel Approaches in Studying Proteasome Assembly and Chaperones.- Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways.- Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis.- Measurement of the Multiple Activities of 26S Proteasomes.- Exploring the Regulation of Proteasome Function by Subunit Phosphorylation.- Scalable In Vitro Proteasome Activity Assay.- Exploring the Rampant Expansion of Ubiquitin Proteomics.- Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome.- Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry.- Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking Assisted Affinity Purification and Mass Spectrometry.
| Erscheinungsdatum | 05.11.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1844 |
| Zusatzinfo | 39 Illustrations, color; 29 Illustrations, black and white; XV, 413 p. 68 illus., 39 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Cul4 • E2 enzymes • flow cytometry • poly-ubiquitin chains • Ubiquitination |
| ISBN-10 | 1-4939-9371-2 / 1493993712 |
| ISBN-13 | 978-1-4939-9371-0 / 9781493993710 |
| Zustand | Neuware |
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