Serpins

Methods and Protocols

Buch | Softcover

276 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-9352-9 (ISBN)

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This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Serpins: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this evolving field.

Overview of Serpins and Their Role in Biological Systems.- Methods for Determining and Understanding Serpin Structure and Function: X-Ray Crystallography.- Serpin Phage Display: Use of a T7 System to Probe Reactive Centre Loop Libraries with Different Serine Proteinases.- Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay.- Methods for Identifying Virus-Derived Serpins.- In Vitro Approaches for the Assessment of Serpin Polymerization.- Cellular Models for the Serpinopathies.- Binding of Serpins to Immobilized Phospholipids and Phospholipids in Suspension.- Viral Serpin Reactive Center Loop (RCL) Peptides: Design and Testing.- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models.- Analysis of In Vivo Serpin Functions in Models of Inflammatory Vascular Disease.- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models.- Serpins in Venous Thrombosis and Venous Thrombus Resolution.- Next Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis after Serpin Treatment.- Methods for Assessing Serpins as Neuroprotective Therapeutics.- Serpins: Development as Clinical Therapeutics.- Serine Protease Assays: Measuring the Enzyme Targets for Serpins, Serine Protease Inhibitors.

Erscheinungsdatum	21.10.2019
Reihe/Serie	Methods in Molecular Biology ; 1826
Zusatzinfo	33 Illustrations, color; 12 Illustrations, black and white; XIII, 276 p. 45 illus., 33 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	drug development • Enzyme inhibition assays • Inhibitory agents • Regulatory drugs • Serine protease inhibitors • Therapeutic design • therapeutics
ISBN-10	1-4939-9352-6 / 1493993526
ISBN-13	978-1-4939-9352-9 / 9781493993529
Zustand	Neuware