Protein Complex Assembly
Humana Press Inc. (Verlag)
978-1-4939-9277-5 (ISBN)
Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Experimental Characterisation Of Protein Complex Structure, Dynamics, and Assembly.- High-Throughput Electron Cryo-Tomography of Protein Complexes and their Assembly.- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single Particle Electron Microscopy.- Time-resolved Cryo-Electron Microscopy Using a Microfluidic Chip.- Characterizing Protein-protein Interactions using Solution NMR Spectroscopy.- Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit For Solution-state and Solid-state NMR.- Characterizing Protein-Protein Interactions using Deep Sequencing Coupled to Yeast Surface Display.- Structurally Guided In Vivo Crosslinking.- Characterising Intact Macromolecular Complexes using Native Mass Spectrometry.- Hydrogen Deuterium Exchange Mass Spectrometry to Study Protein Complexes.- Structural Analysis of Protein Complexes by Cross-linking and Mass-spectrometry.- Global Characterization of Protein Complexes by Biochemical Purification-mass Spectrometry (BP/MS).- Proteomic Profiling of Integrin Adhesion Complex Assembly.- Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies.- Correlative 3D Structured Illumination Microscopy and Single Molecular Localisation Microscopy for Imaging Cancer Invasion.- Observing the Assembly of Protein Complexes In Living Eukaryotic Cells In Super-resolution using refSOFI.- Detecting Purinosome Metabolon Formation with Fluorescence Microscopy.- Analysis of Bacterial pilus Assembly by Shearing and Immunofluorescence Microscopy.- Expression, Purification and Assembly of Archaellum Subcomplexes of Sulfolobus Acidocaldarius.- Unstable Protein Purification through the Formation of Stable Complexes.- Expressing Multi-subunit Complexes using biGBac.- Computational Modelling of Protein Complex Structure and Assembly.- Inferring and using Protein Quaternary Structure Information from Crystallographic.- Searching and Extracting Data from the EMBL-EBIComplex Portal.- Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-Purification Data.- A Multi-scale Computational Model for Simulating the Kinetics of Protein Complex Assembly.- Flexible Protein-protein Docking with SwarmDock.- Protein-protein Docking using Evolutionary Information.- Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles.- Modeling the Structure of Helical Assemblies with Experimental Constraints in.- Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ).
| Erscheinungsdatum | 15.05.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1764 |
| Zusatzinfo | 79 Illustrations, color; 14 Illustrations, black and white; XV, 506 p. 93 illus., 79 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Adhesion • co-translational assembly • Cryoelectron microscopy • Mass Spectrometry • stoichiometry |
| ISBN-10 | 1-4939-9277-5 / 1493992775 |
| ISBN-13 | 978-1-4939-9277-5 / 9781493992775 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
aus dem Bereich
