Functional Disulphide Bonds
Humana Press Inc. (Verlag)
978-1-4939-9186-0 (ISBN)
Authoritative and practical, FunctionalDisulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study.
1. Classification of protein disulfide bonds.- 2. Assessing the evolutionary conservation of protein disulphide bonds.- 3. A proteomics workflow for the Identification of labile disulphide bonds at the cell surface.- 4. Quantification of the redox state of protein disulfide bonds.- 5. Quantification of the redox state of protein disulfide bonds.- 6. Studying functional disulfide bonds by computer simulations.- 7. Dynamic force spectroscopy analysis on the redox states of protein disulfide bonds.- 8. Assays of thiol isomerase enzymatic activity.- 9. Functional assays of thiol isomerase ERp5.- 10. IDENTIFICATION OF PDI SUBSTRATES BY MECHANISM-BASED KINETIC TRAPPING.- 11. Site-specific proteomic mapping of modified cysteine residues.- 12. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry.- 13. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry.- 14. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide.- 15. Methodology for identification of the cysteine-reactive covalent inhibitors.- 16. In Vivo Measurement of Redox-Regulated TG2 Activity.- 17. Quantitation of total and free thiol β2-glycoprotein I levels for diagnostic and prognostic purposes in the antiphospholipid syndrome.- 18. Quantitation of total and free thiol angiotensinogen as a prognostic marker for pre-eclampsia.- 19. Preparation of a dithiol-reactive probe for PET imaging of cell death.- 20. Flow Cytometry Assessment of Procoagulant Platelets Using A Dithiol Reactive Probe.
Erscheinungsdatum | 24.05.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1967 |
Zusatzinfo | 69 Illustrations, color; 55 Illustrations, black and white; XI, 327 p. 124 illus., 69 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Covalent drugs • Cysteine thiols • Polypeptide chains • Post-translational modification • Protein function |
ISBN-10 | 1-4939-9186-8 / 1493991868 |
ISBN-13 | 978-1-4939-9186-0 / 9781493991860 |
Zustand | Neuware |
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