The Extracellular Matrix

Methods and Protocols

Davide Vigetti, Achilleas D. Theocharis (Herausgeber)

Buch | Hardcover

278 Seiten

2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9132-7 (ISBN)

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This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.

The Complex Interplay between Extracellular Matrix and Cells in Tissues.- Method for Studying ECM Expression: In Situ RT-PCR.- Visualizing the Supramolecular Assembly of Collagen.- Steady-state and Pulse-chase Analyses of Fibrillar Collagen.- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates.- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems.- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples.- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis.- Hyaluronan Pericellular Matrix: Particle Exclusion Assay.- Determination of Cell Surface Hyaluronan through Flow Cytometry.- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine.- Analyzing Hyaluronidases in Biological Fluids.- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent IonChannels Gating.- Methods for Monitoring Matrix-Induced Autophagy.- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography.- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography.- In Vitro Spheroid Sprouting Assay of Angiogenesis.- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization.- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding.- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment.

Erscheinungsdatum	22.03.2019
Reihe/Serie	Methods in Molecular Biology ; 1952
Zusatzinfo	27 Illustrations, color; 22 Illustrations, black and white; XII, 278 p. 49 illus., 27 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	cell-matrix interactions • Drug Delivery • ECM molecules • exosomes • Matrix biology • Tissue surrogates
ISBN-10	1-4939-9132-9 / 1493991329
ISBN-13	978-1-4939-9132-7 / 9781493991327
Zustand	Neuware