Protein Engineering
Humana Press Inc. (Verlag)
978-1-4939-8463-3 (ISBN)
Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.
The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
Protein Engineering - Past, Present, and Future.- (Semi)rational Protein Design.- Computational Analysis of Protein Tunnels and Channels.- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations.- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO.- Directed Evolution of Proteins Based on Mutational Scanning.- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries.- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers.- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification.- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation.- Practical Considerations Regarding the Choice of the Best High-throughput Assay.- High-throughput Screening Assays for Lipolytic Enzymes.- Continuous High-throughput Colorimetric Assays for α-transaminases.-Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases.- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions.- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation.- Solid Phase Agar Plate Assay for Screening Amine Transaminases.- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics.- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display.- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles.
| Erscheinungsdatum | 27.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1685 |
| Zusatzinfo | 63 Illustrations, color; 19 Illustrations, black and white; XI, 350 p. 82 illus., 63 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| ISBN-10 | 1-4939-8463-2 / 1493984632 |
| ISBN-13 | 978-1-4939-8463-3 / 9781493984633 |
| Zustand | Neuware |
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