Noncanonical Amino Acids
Humana Press Inc. (Verlag)
978-1-4939-7573-0 (ISBN)
Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.
Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates.- Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis.- Cell-free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 deletion E. coli Strains.- Tub-tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids.- Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids.- Genetic Code Expansion in Enteric Bacterial Pathogens.- Self-directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and its Incorporation into Model Proteins.- Residue-specific Incorporation of no Canonical Amino Acids for Protein Engineering.- Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene.- Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439.- Expression and Purification of Site-specifically Lysine-acetylated and Natively-folded Proteins for Biophysical Investigations.- Site-specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code.- Site-specific Protein Labeling With Tetrazine Amino Acids.- Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers.- Generation of Stable Amber Suppression Cell Lines.- Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo.- Genetically Encoding Unnatural Amino Acids in Neurons in vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins.- Genetic Code Expansion-and Click Chemistry-Based Site--Specific Protein Labelling For Intracellular DNA--PAINT Imaging.- MultiBacTAG- Genetic Code Expansion using the Baculovirus Expression System in Sf21 Cells.- Production and Chemoselective Modification of Adeno-associated Virus Site-specifically Incorporating an Unnatural Amino Acid Residue into its Capsid.- Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis.- Fluorogenic Tetrazine--siliconrhodamine Probe for the Labeling of Non-canonical Amino Acid Tagged Proteins.- Site-specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction.- Genetic Encoding of Unnatural Amino Acids in C. elegans.
| Erscheinungsdatum | 26.02.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1728 |
| Zusatzinfo | 39 Illustrations, color; 37 Illustrations, black and white; XI, 411 p. 76 illus., 39 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Schlagworte | Drosophila • E. coli • Tag • tRNA • Xenopus |
| ISBN-10 | 1-4939-7573-0 / 1493975730 |
| ISBN-13 | 978-1-4939-7573-0 / 9781493975730 |
| Zustand | Neuware |
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