Liver Proteomics

Methods and Protocols

Djuro Josic, Douglas C. Hixson (Herausgeber)

Buch | Softcover

346 Seiten

2016 | Softcover reprint of the original 1st ed. 2012
Humana Press Inc. (Verlag)
978-1-4939-6214-3 (ISBN)

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The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

Cell Surface Capturing (CSC) technologies for the surfaceome discovery of hepatocytes.-An on-target desalting and concentration sample preparationprotocol for MALDI-MS and MS/MS analysis.-Plasma membrane isolation using immobilized concanavalin A magnetic beads.-Analysis of the cattle liver proteome by High-sensitive Liquid Chromatography Coupled with Mass Spectrometry method.-Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450.-Assessing heterogeneity of peroxisomes – isolation of two subpopulations from rat liver.-Purification and Proteomic Analysis of Liver Membrane Skeletons.-Liver Plasma Membranes: An effective method to analyze membrane proteome.-Quantitative analysis of liver Golgi proteome in the cell cycle.-Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by top-down MALDI-TOF Mass Spectrometry.-Phosphoproteomic Analysis of Liver Homogenates.-A combination of affinity chromatography, 2D DIGE and mass spectrometry to analyze the phosphoproteome of liver progenitor cells.-Identifying Acetylated Proteins in Mitosis.-Comparative proteome analysis of a human liver cell line stably transfected with Hepatitis D virus full-length cDNA.-Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology.-Proteomics analysis of human non-alcoholic fatty liver.-Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis.-Targeted mass spectrometry-based metabolomic profiling through Multiple Reaction Monitoring of Liver and other biological matrices.-Discovery of Lamin B1 and Vimentin as CirculatingBiomarkers for Early Hepatocellular Carcinoma.-Combinatorial peptide ligand libraries to discover liver disease biomarkers in plasma samples.-Isolation of urinary exosomes from animal models to unravel non-invasive disease biomarker.

Erscheinungsdatum	19.08.2017
Reihe/Serie	Methods in Molecular Biology ; 909
Zusatzinfo	XIII, 346 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	1-4939-6214-0 / 1493962140
ISBN-13	978-1-4939-6214-3 / 9781493962143
Zustand	Neuware