Liver Proteomics
Humana Press Inc. (Verlag)
978-1-61779-958-7 (ISBN)
The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.
Cell Surface Capturing (CSC) technologies for the surfaceome discovery of hepatocytes.-An on-target desalting and concentration sample preparationprotocol for MALDI-MS and MS/MS analysis.-Plasma membrane isolation using immobilized concanavalin A magnetic beads.-Analysis of the cattle liver proteome by High-sensitive Liquid Chromatography Coupled with Mass Spectrometry method.-Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450.-Assessing heterogeneity of peroxisomes – isolation of two subpopulations from rat liver.-Purification and Proteomic Analysis of Liver Membrane Skeletons.-Liver Plasma Membranes: An effective method to analyze membrane proteome.-Quantitative analysis of liver Golgi proteome in the cell cycle.-Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by top-down MALDI-TOF Mass Spectrometry.-Phosphoproteomic Analysis of Liver Homogenates.-A combination of affinity chromatography, 2D DIGE and mass spectrometry to analyze the phosphoproteome of liver progenitor cells.-Identifying Acetylated Proteins in Mitosis.-Comparative proteome analysis of a human liver cell line stably transfected with Hepatitis D virus full-length cDNA.-Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology.-Proteomics analysis of human non-alcoholic fatty liver.-Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis.-Targeted mass spectrometry-based metabolomic profiling through Multiple Reaction Monitoring of Liver and other biological matrices.-Discovery of Lamin B1 and Vimentin as CirculatingBiomarkers for Early Hepatocellular Carcinoma.-Combinatorial peptide ligand libraries to discover liver disease biomarkers in plasma samples.-Isolation of urinary exosomes from animal models to unravel non-invasive disease biomarker.
| Erscheint lt. Verlag | 18.8.2012 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 909 |
| Zusatzinfo | XIII, 346 p. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Studium |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| ISBN-10 | 1-61779-958-0 / 1617799580 |
| ISBN-13 | 978-1-61779-958-7 / 9781617799587 |
| Zustand | Neuware |
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