Potassium Channels
Humana Press Inc. (Verlag)
978-1-4939-7361-3 (ISBN)
Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels.
Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation.- Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes.- Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane using Surface Biotinylation.- Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome.- Probing Subunits Interactions in KATP Channels using Photo-Crosslinking via Genetically Encoded p-azido-L-phenylalanine.- Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization.- Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function.- Methods for Characterizing Disease-Associated ATP-SensitivePotassium Channel Mutations.- Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters.- Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions.- Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes.- Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations.- Studying Structural Dynamics of the Potassium Channels by Single-Molecule FRET.- Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters.- Combinatorial Assembly of Lumitoxins.- Characterization of MC4R Regulation of the Kir7.1 Channel using the Tl+ Flux Assay.- Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies.- In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish.- Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevisOocytes.- Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels.- CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels.- Ion Binding to Transport Proteins using Isothermal Titration Calorimetry.- Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling.- Studying Kv Channels Function using Computational Methods.
| Erscheinungsdatum | 31.10.2017 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1684 |
| Zusatzinfo | 53 Illustrations, color; 31 Illustrations, black and white; XVI, 345 p. 84 illus., 53 illus. in color. With online files/update. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Channelopathies • channel structure • ion channels • membrane excitability • Patching K+ channels • pharmacology |
| ISBN-10 | 1-4939-7361-4 / 1493973614 |
| ISBN-13 | 978-1-4939-7361-3 / 9781493973613 |
| Zustand | Neuware |
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