Selenoproteins
Humana Press Inc. (Verlag)
978-1-4939-7257-9 (ISBN)
Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins.- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins.- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly.- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing.- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs.- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis.- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling.- Modification of Selenoprotein mRNAs by Cap Tri-methylation.- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS).-Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum.- Radioactive 75Se Labeling and Detection of Selenoproteins.- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines.- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips.- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues.- Overexpression of Recombinant Selenoproteins in E. coli.- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS.- Selenocysteine Mediated Expressed Protein Ligation of SELENOM.- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity.- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities.- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk.- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis.
| Erscheinungsdatum | 22.10.2017 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1661 |
| Zusatzinfo | 35 Illustrations, color; 28 Illustrations, black and white; XV, 340 p. 63 illus., 35 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Binding Proteins • biosynthesis • redox enzymes • SECIS • TrxRD |
| ISBN-10 | 1-4939-7257-X / 149397257X |
| ISBN-13 | 978-1-4939-7257-9 / 9781493972579 |
| Zustand | Neuware |
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