JAK-STAT Signalling
Humana Press Inc. (Verlag)
978-1-4939-5979-2 (ISBN)
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation.- Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins.- In Vitro JAK Kinase Activity and Inhibition Assays.- Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance.- Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach.- Designing RNAi Screens to Identify JAK/STAT Pathway Components.- The Use of JAK-Specific Inhibitors as Chemical Biology Tools.- Methods for Detecting Mutations in the Human JAK2 Gene.- Detection of Activated STAT Proteins.- Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents.- Flow Cytometric Analysis of STAT Phosphorylation.- Acetylation of Endogenous STAT Proteins.- Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining.- Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging.- Characterization of STAT Self-Association by Analytical Ultracentrifugation.- Constitutively Active STAT5 Constructs.- Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR.- Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis.- In vitro Ubiquitination of Cytokine Signaling Components.- Production and Crystallization of Recombinant JAK Proteins.- Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins.
Erscheinungsdatum | 24.07.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 967 |
Zusatzinfo | XI, 320 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-4939-5979-4 / 1493959794 |
ISBN-13 | 978-1-4939-5979-2 / 9781493959792 |
Zustand | Neuware |
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