Bioluminescence
Humana Press Inc. (Verlag)
978-1-4939-3811-7 (ISBN)
This volume
presents detailed laboratory protocols regarding the three major route
technologies luciferases, efficient optical
probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful
complement to the first and second edition for new and experienced researchers
alike.
Label-free Cell Phenotypic Identification
of D-Luciferin as an Agonist for GPR35.- Synthetic Bioluminescent
Coelenterazine Derivatives.- Molecular Cloning of Secreted
Luciferases from Marine Planktonic Copepods.- How to Fabricate Functional
Artificial Luciferases for Bioassays.- Quantum
Yield Determination Based on Photon Number Measurement, Protocols for Firefly
Bioluminescence Reactions.- Bioluminescent Ligand-Receptor
Binding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of Aminopeptidase
N in vitro and in vivo.- Firefly Luciferase-based
Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence
Resonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Change
with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and
Mammalian Cells.- A Protein–Protein Interaction
Assay FlimPIA Based on the Functional Complementation of Mutant Firefly
Luciferases.- Single-chain Probes for Illuminating
Androgenicity of Chemicals.- Multicolor
Imaging of Bifacial Activities of Estrogens.- Circular
Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor.- Fabrication
of Molecular Strain Probes for Illuminating Protein–Protein Interactions.- An
ALuc-based Molecular Tension Probe for Sensing Intramolecular Protein–Protein Interactions.- Live Cell Bioluminescence Imaging
in Temporal Reaction of G Protein-coupled Receptor for High-throughput Screening
and Analysis.- Imaging Histone Methylations in
Living Animals.- Preparation and Assay of Simple Light off Biosensor Based on Immobilized
Bioluminescent Bacteria for General Toxicity Assays.- In
Vivo Bioluminescent Imaging of ATP-Binding
Cassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of Cancer
Gene Therapy.- Development of a Multicolor Bioluminescence
Imaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signaling
and Apoptosis .- A Multichannel Bioluminescence
Determination platform for Bioassays.- A Bioluminescence Assay System
for Imaging Metal Cationic Activities in Urban Aerosols.- Luminescence
Imaging: (a) Multi-color Visualization of Ca2+ Dynamics in Different
Cellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.- Photon Counting System for High-sensitivity
Detection of Bioluminescence at Optical Fiber End.
| Erscheinungsdatum | 08.10.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1461 |
| Zusatzinfo | 84 Illustrations, color; 16 Illustrations, black and white; XIII, 314 p. 100 illus., 84 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Schlagworte | de novo luciferases • Luciferase • molecular events • optical performance • optical probes |
| ISBN-10 | 1-4939-3811-8 / 1493938118 |
| ISBN-13 | 978-1-4939-3811-7 / 9781493938117 |
| Zustand | Neuware |
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