Proteostasis
Humana Press Inc. (Verlag)
978-1-4939-3754-7 (ISBN)
Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.
UPS Activation in the Battle against Aging and Aggregation-Related
Diseases: An Extended Review.- Review
And Literature Mining On Proteostasis Factors’ And Cancer.- Combining Zebrafish and Mouse Models to Test the
Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina.- Immunodepletion and Immunopurification as Approaches
for CSN Research.- Studying Protein Ubiquitylation in Yeast.- Strategies to Detect Endogenous Ubiquitination of a
Target Mammalian Protein.- In Vitro Ubiquitination – Self-Ubiquitination, Chain
Formation, and Substrate Ubiquitination Assays.- Isolation of the Ubiquitin-Proteome From Tumour Cell
Lines and Primary Cells Using Tubes.- Tubes-Mass Spectrometry for Identification and
Analysis of the Ubiquitin-Proteome.- Isolation of Ubiquitinated Proteins to High
Purity from In Vivo Samples.- Method for the Purification of Endogenous Unanchored
Polyubiquitin Chains.- Fluorescent Tools for In Vivo Studies on
the Ubiquitin-Proteasome System.- Bimolecular Fluorescence Complementation to Assay
the Interactions of Ubiquitylation Enzymes in Living Yeast Cells.- Monitoring Ubiquitin-Coated Bacteria Via Confocal
Microscopy.- Detection and Analysis of Cell Cycle-Associated
Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo.- Detection and Analysis Of Sumoylation Substrates In
Vitro and In Vivo. - Detection of Protein-Protein Interactions and
Post-Translational Modifications using the Proximity Ligation Assay:
Application to the Study of the Sumo Pathway.- Dissecting of Sumo Dynamics by Mass Spectrometry.- Isolation of Lysosomes from Mammalian Tissues and
Cultured Cells.- Analysis Of Relevant Parameters For Autophagic Flux
Using Hela Cells Expressing Egfp-Lc3.- Analysis of Protein Oligomeric Species by Sucrose
Gradients.- Analysis of
Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool
to Identify Oxidized Proteins.- Quantitation of
Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry.- A Simple Protocol for High Efficiency Protein
Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue
Samples.- Monitoring Target Engagement Of Deubiquitylating
Enzymes Using Activity Probes: Past,
Present, and Future.- Activity Based Profiling of Deubiquitylating Enzymes
and Inhibitors in Animal Tissues.- High-Throughput Sirna Screening Applied to the
Ubiquitin-Proteasome System.- High-Throughput Yeast-Based Reporter Assay to
Identify Compounds with Anti-Inflammatory Potential.- Using
Alphascreen ® to Identify Small Molecule Inhibitors Targeting a
Conserved Host-Pathogen Interaction.- Global MS-Based Proteomics Drug Profiling.
| Erscheinungsdatum | 22.10.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1449 |
| Zusatzinfo | 60 Illustrations, color; 34 Illustrations, black and white; XVI, 488 p. 94 illus., 60 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | disease model systems • Human health • human proteins • protein homeostasis • target proteostasis for therapeutics |
| ISBN-10 | 1-4939-3754-5 / 1493937545 |
| ISBN-13 | 978-1-4939-3754-7 / 9781493937547 |
| Zustand | Neuware |
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