Bioconjugate Techniques
Seiten
1996
Academic Press Inc (Verlag)
978-0-12-342335-1 (ISBN)
Academic Press Inc (Verlag)
978-0-12-342335-1 (ISBN)
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A guide to the modification and crosslinking of biomolecules for use in research, diagnostics and therapeutics. The text provides detailed information on the chemistry, reagent systems, and practical applications for creating labelled or conjugate molecules.
This is a guide to the modification and crosslinking of biomolecules for use in research, diagnostics and therapeutics. It provides detailed information on the chemistry, reagent systems, and practical applications for creating labelled or conjugate molecules. It also describes a range of reactions with details on commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids and synthetic polymers. This text is intended to help readers form unique complexes that can be used for detecting, quantifying and targeting important analytes. It is aimed at researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology and microbiology.
This is a guide to the modification and crosslinking of biomolecules for use in research, diagnostics and therapeutics. It provides detailed information on the chemistry, reagent systems, and practical applications for creating labelled or conjugate molecules. It also describes a range of reactions with details on commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids and synthetic polymers. This text is intended to help readers form unique complexes that can be used for detecting, quantifying and targeting important analytes. It is aimed at researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology and microbiology.
Part 1 Bioconjugate chemistry: functional targets; the chemistry of reactive groups. Part 2 Bioconjugate reagents: zero-length cross-linkers; homobifunctional cross-linkers; heterobifunctional cross-linkers; trifunctional cross-linkers; cleavable reagent systems; tags and probes. Part 3 Bioconjugate applications: preparation of hapten-carrier immunogen conjugates; antibody modification and conjugation; immunotoxin conjugation techniques; preparation of liposome conjugates and derivatives; avidin-biotin systems; preparation of colloidal gold labelled proteins; modification with synthetic polymers; enzyme modification and conjugation; nucleic acid and oligonucleotide modification and conjugation.
| Erscheint lt. Verlag | 1.1.1996 |
|---|---|
| Zusatzinfo | illustrations |
| Verlagsort | San Diego |
| Sprache | englisch |
| Maße | 182 x 259 mm |
| Gewicht | 1525 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-12-342335-X / 012342335X |
| ISBN-13 | 978-0-12-342335-1 / 9780123423351 |
| Zustand | Neuware |
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