Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
Humana Press Inc. (Verlag)
978-1-4939-1141-7 (ISBN)
Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.
Twelve Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC).- Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture.- SILAC Labeling of Yeast for the Study of Membrane Protein Complexes.- Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry.- Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons.- SILAC and Alternatives in Studying Cellular Proteomes of Plants.- In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster.- Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse.- Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans.- SILAC-based Temporal Phosphoproteomics.- Global Ubiquitination Analysis by SILAC in Mammalian Cells.- Quantifying In Vivo, Site-specific Changes in Protein Methylation with SILAC.- Applying SILAC for the Differential Analysis of Protein Complexes.- Defining Dynamic Protein Interactions Using SILAC-based Quantitative Mass Spectrometry.- Identifying Nuclear Protein-Protein Interactions using GFP Affinity Purification and SILAC-based Quantitative Mass Spectrometry.- Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC.- Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling.- Protein Correlation Profiling-SILAC to Study Protein-Protein Interactions.- Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC.- Design and Application of Super-SILAC for Proteome Quantification.- Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies.- Analysis of Secreted Proteins using SILAC.- Identification of MicroRNA Targets by Pulsed SILAC.- MaxQuant forIn-Depth Analysis of Large SILAC Datasets.
Reihe/Serie | Methods in Molecular Biology ; 1188 |
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Zusatzinfo | 27 Illustrations, color; 38 Illustrations, black and white; XIV, 372 p. 65 illus., 27 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Chemie ► Analytische Chemie | |
Schlagworte | autotrophic organisms • Gram negative bacteria • Gram positive bacteria • human protein complexes • human splicosome • metabolic labeling • nuclear protein-protein interactions • protein interactomes • quantitative phosphoproteome analyses in C. elegans • RNAi-mediated gene knockdown • Signaling networks • SILAC labeling of the yeast Saccharomyces cerevisiae • SILAC technology • time-resolved phosphoproteomics studies • Trypanosoma brucei |
ISBN-10 | 1-4939-1141-4 / 1493911414 |
ISBN-13 | 978-1-4939-1141-7 / 9781493911417 |
Zustand | Neuware |
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