Basic Cloning Procedures
Seiten
2012
|
1998
Springer Berlin (Verlag)
978-3-642-48977-8 (ISBN)
Springer Berlin (Verlag)
978-3-642-48977-8 (ISBN)
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.
| Erscheint lt. Verlag | 7.7.2012 |
|---|---|
| Reihe/Serie | Springer Lab Manuals |
| Zusatzinfo | VII, 169 p. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 284 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | DNA • Gene • Nucleic acid • Protein • Protein synthesis • RNA • sequencing • synthesis |
| ISBN-10 | 3-642-48977-X / 364248977X |
| ISBN-13 | 978-3-642-48977-8 / 9783642489778 |
| Zustand | Neuware |
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