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Membrane Proteins

Folding, Association, and Design

Giovanna Ghirlanda, Alessandro Senes (Herausgeber)

Buch | Hardcover

246 Seiten

2013
Humana Press Inc. (Verlag)
978-1-62703-582-8 (ISBN)

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Focusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems. The volume is divided into four detailed sections, covering association of transmembrane helices, interactions with the lipid bilayer, NMR methods, as well as a variety of engineering approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Membrane Proteins: Folding, Association, and Design serves as an ideal guide for researchers reaching for the tantalizing possibility of designing novel membrane proteins with tailored functionality.

Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments.- Measurement of Transmembrane Peptide Interactions in Liposomes Using Förster Resonance Energy Transfer (FRET).- Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping.- Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes.- Analyzing the Effects of Hydrophobic Mismatch on Transmembrane α-Helices Using Tryptophan Fluorescence Spectroscopy.- Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure.- Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes.- Membrane Protein Structure Determination: Back to the Membrane.- On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides.- Prediction and Design of Outer Membrane Protein-Protein Interactions.- Design of Transmembrane Peptides: Coping with Sticky Situations.- Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors.- Fluorination in the Design of Membrane Protein Assemblies.

Reihe/Serie	Methods in Molecular Biology ; 1063
Zusatzinfo	37 Illustrations, color; 24 Illustrations, black and white; X, 246 p. 61 illus., 37 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Functional artificial proteins • lipid bilayer • NMR Methods • Protein Engineering • Protein Folding • protein-protein interactions • Transmembrane peptides
ISBN-10	1-62703-582-6 / 1627035826
ISBN-13	978-1-62703-582-8 / 9781627035828
Zustand	Neuware