Green Fluorescent Proteins
John Wiley & Sons Inc (Verlag)
978-0-471-17839-2 (ISBN)
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Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include: The discovery of GFP Bioluminescence and biofluorescence in nature Biochemical and physical properties of GFP The three-dimensional structure of GFP and its implications for function and design Molecular biology and modification of GFP Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites
BACKGROUND.; The Discovery of Green Fluorescent Protein.; Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems.; BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN.; Biochemical and Physical Properties of Green Fluorescent Protein.; The Three--Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design.; Molecular Biology and Mutation of Green Fluorescent Protein.; APPLICATIONS OF GREEN FLUORESCENT PROTEIN.; The Uses of Green Fluorescent Protein in Caenorhabditis elegans.; The Uses of Green Fluorescent Protein in Prokaryotes.; The Uses of Green Fluorescent Protein in Yeasts.; The Uses of Green Fluorescent Protein in Drosophila.; The Uses of Green Fluorescent Protein in Plants.; The Uses of Green Fluorescent Protein in Trangenic Vertebrates.; The Uses of Green Fluorescent Protein in Mammalian Cells.; METHODS AND PROTOCOLS.; Expressioin of Green Fluorescent Protein.; Specimen Preparation.; Visualization of Green Fluorescent Protein.; Documenting Green Fluorescent Protein.; Appendix.; Index.
| Erscheint lt. Verlag | 24.9.1998 |
|---|---|
| Zusatzinfo | Illustrations (some col.) |
| Verlagsort | New York |
| Sprache | englisch |
| Maße | 179 x 253 mm |
| Gewicht | 737 g |
| Einbandart | Paperback |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| ISBN-10 | 0-471-17839-X / 047117839X |
| ISBN-13 | 978-0-471-17839-2 / 9780471178392 |
| Zustand | Neuware |
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