Hormonally Defined Media

A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982

G. Fischer, R. J. Wieser (Herausgeber)

Buch | Softcover

XIV, 458 Seiten

2011 | 1. Softcover reprint of the original 1st ed. 1983
Springer Berlin (Verlag)
978-3-642-69292-5 (ISBN)

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Until some years ago serum or crude tissue extracts were used pre dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re gard to survival, proliferation and differentiation of cells in cul ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.

Opening Lecture.- Nutritional Requirements.- Cell Proliferation and Growth Factors.- Cell-Substratum Interactions.- Neural Cells.- Endocrine and Exocrine Cells.- Hemopoietic and Immune System.- Liver- and Kidney-Derived Cells.- Muscle Cells, Epithelial Cells, Fibroadipogenic Cells, Melanocytes and Chondrocytes.- Tumor Cells.

Erscheint lt. Verlag	7.12.2011
Reihe/Serie	Proceedings in Life Sciences
Zusatzinfo	XIV, 458 p.
Verlagsort	Berlin
Sprache	englisch
Maße	170 x 244 mm
Gewicht	813 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Biology • Cell • Cell Biology • Cell Culture • Fischer • Hormonhaltiger serumfreier Nährboden • Influence • tissue • Zellkultur
ISBN-10	3-642-69292-3 / 3642692923
ISBN-13	978-3-642-69292-5 / 9783642692925
Zustand	Neuware