Methods of Protein and Nucleic Acid Research

Volume 1: Electrophoresis · Isoelectric Focusing Ultracentrifugation

L.A. Osterman (Autor)

Buch | Softcover

X, 344 Seiten

2012 | Softcover reprint of the original 1st ed. 1984
Springer Berlin (Verlag)
978-3-642-87487-1 (ISBN)

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Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.

I Electrophoresis.- 1 Gels for Electrophoresis.- 2 Techniques of Gel Preparation and Necessary Equipment.- 3 Electrophoresis of Proteins.- 4 Electrophoresis of Nucleic Acids.- II Isoelectric Focusing (IEF).- 1 Principle of the Method.- 2 Analytical Variations of IEF.- 3 Two-Dimensional Fractionation of Proteins.- 4 Preparative Variants of IEF.- 5 Isotachophoresis.- III Ultracentrifugation.- 1 Sedimentation - Basic Concepts.- 2 Ultracentrifuge.- 3 Rotors and Tubes.- 4 Differential Centrifugation (Pelleting).- 5 Rate-Zonal Centrifugation.- 6 Equilibrium (Isopycnic) Centrifugation.- 7 Centrifugation in Zonal Rotors.- References to Part I.- References to Part II.- References to Part III.- List of Selected Examples.- Nomograms for Calculation of Centrifugation Time (for Practical Use).

Erscheint lt. Verlag	13.4.2012
Zusatzinfo	X, 344 p.
Verlagsort	Berlin
Sprache	englisch
Maße	170 x 244 mm
Gewicht	614 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Chemie ► Analytische Chemie
Schlagworte	Elektrophorese • Isoelektrische Fokussierung • Protein • Protein / Eiweiß • Ultrazentrifugation
ISBN-10	3-642-87487-8 / 3642874878
ISBN-13	978-3-642-87487-1 / 9783642874871
Zustand	Neuware