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Protein Purification -

Protein Purification

Principles, High Resolution Methods and Applications
Buch | Hardcover
712 Seiten
1997 | 2nd Revised edition
John Wiley & Sons Inc (Verlag)
978-0-471-18626-7 (ISBN)
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This book is a state-of-the-art treatise of modern, high resolution biochemical separation techniques for proteins. It covers all major methods in chromatography and electrophoresis in a format which makes it readily accessible to university students and practitioners alike.
Praise for the First Edition of Protein Purification: "Contains much useful information for both the novice in protein purification and the experienced practitioner ...[an] excellent practical manual." -Trends in Analytical Chemistry. "[This book is] easy to read and gives a comprehensive description of all major methods of protein purification ...a much welcomed source of information for protein biochemists." -FEBS Letters. This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography Reversed phase and hydrophobic interaction chromatography Electrophoresis in gels Immunoelectrophoresis and capillary electrophoresis Protein mapping by two-dimensional polyacrylamide gel electrophoresis Protein elution and blotting methods Large-scale electrophoresis processes.

JAN--CHRISTER JANSON is Adjunct Professor of Technical Biochemistry at Uppsala University and Principal Scientist at Amersham Pharmacia Biotech in Sweden. LARS RYDEN is Assistant Professor of Biochemistry at Uppsala University in Sweden.

Introduction to Protein Purification (B. Ersson, et al.).; CHROMATOGRAPHY.; Introduction to Chromatography (J.--C.; Janson & J.--A.; Jonsson).; Gel Filtration (L. Hagel).; Ion Exchange Chromatography (E. Karlsson, et al.).; Chromatofocusing (T. Hutchens).; High--Resolution Reversed--Phase Chromatography (M. Hearn).; Hydrophobic Interaction Chromatography (K.--O.; Eriksson).; Immobilized Metal Ion Affinity Chromatography (L. Kagedal).; Covalent Chromatography (J. Carlsson, et al.).; Affinity Chromatography (J. Carlsson, et al.).; Affinity Partitioning of Proteins Using Aqueous Two--Phase Systems (G. Johansson).; ELECTROPHORESIS.; Electrophoresis in Gels (T. Laas).; Isoelectric Focusing (T. Laas).; Immunoelectrophoresis (J. Lizana).; Protein Mapping by Two--Dimensional Polyacrylamide Electrophoresis (R. Westermeier & A. Gorg).; Protein Elution and Blotting Techniques (R. Westermeier).; Capillary Electrophoretic Separations (W. Thormann).; Large--Scale Electrophoretic Processes (W. Thormann).; Index.

Erscheint lt. Verlag 24.3.1998
Zusatzinfo figures
Verlagsort New York
Sprache englisch
Maße 162 x 242 mm
Gewicht 1086 g
Einbandart gebunden
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Chemie Analytische Chemie
Naturwissenschaften Chemie Organische Chemie
ISBN-10 0-471-18626-0 / 0471186260
ISBN-13 978-0-471-18626-7 / 9780471186267
Zustand Neuware
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