Macromolecular Crystallography Protocols, Volume 1

Preparation and Crystallization of Macromolecules

Buch | Softcover

280 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2007
Humana Press Inc. (Verlag)
978-1-61737-475-3 (ISBN)

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Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag.- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project.- Baculoviral Expression of an Integral Membrane Protein for Structural Studies.- Protein Engineering.- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems.- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals.- Screening and Optimization Methods for Nonautomated Crystallization Laboratories.- Improving Marginal Crystals.- Optimization Techniques for Automation and High Throughput.- Three-Dimensional Crystallization of Membrane Proteins.- Crystallization of Protein-DNA Complexes.- Preparation and Crystallization of RNA.- Crystallization of RNA-Protein Complexes.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Biology ; 363
Zusatzinfo	49 Illustrations, black and white; XIV, 280 p. 49 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-475-X / 161737475X
ISBN-13	978-1-61737-475-3 / 9781617374753
Zustand	Neuware