Surface Plasmon Resonance
Humana Press Inc. (Verlag)
978-1-60761-669-6 (ISBN)
Surface plasmon resonance (SPR) has evolved into an exciting technique in biomolecular interaction analysis. The development of commercial SPR instruments has made the te- nique available to a wide scienti?c audience, and the number of publications in which the use of SPR is described is rapidly increasing. SPR is in use for many purposes from food quality control to the study of nanoparticles. Much research is now focused on devel- ing new SPR-related applications, e.g., SPR imaging, SPR arrays, SPR ?uorescence, and combinations of SPR with mass spectrometry and with electrochemistry. Biomolecular interaction analysis is at the core of many research projects. In principle, the setup of an SPR experiment is simple: There is a sensor surface to which one of the interacting partners (the ligand) is immobilized; the other partner (the analyte) is added in a ?ow or cell-like compartment. The binding phenomenon is monitored in real time as a change in SPR angle. An important issue is the choice of surface and the immobilization strategy. With SPR, it is possible to mimic the biological environment which is relevant for an interaction.
For interactions in a water environment, sensor surfaces with hydrogels are available. Many biomolecular interactions take place in a membrane environment. For this, commercial sensor surfaces are available, or surfaces can be tailor-made. This volume contains several examples of building up of lipophilic surfaces. Nature abundantly makes use of multivalent interactions; multivalency can be mimicked on a sensor surface with immobilized ligands.
Surface Plasmon Resonance: A General Introduction.- The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing.- Amine Coupling Through EDC/NHS: A Practical Approach.- High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies.- A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance.- Affinity Constants for Small Molecules from SPR Competition Experiments.- Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules.- High-Throughput Kinase Assay Based on Surface Plasmon Resonance.- SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads.- Carbohydrate–Lectin Interactions Assayed by SPR.- DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films.- Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors.- Capture of Intact Liposomes on Biacore Sensor Chips for Protein–Membrane Interaction Studies.- Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides.- Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid ? Proteins (A?) and Lipid Membranes.- Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies.- Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling.- Integration of SPR Biosensors with Mass Spectrometry (SPR-MS).- SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry.
| Reihe/Serie | Methods in Molecular Biology ; 627 |
|---|---|
| Zusatzinfo | X, 286 p. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Naturwissenschaften ► Chemie ► Analytische Chemie | |
| Schlagworte | Binding modes • Biomolecular interaction analysis • Hydrogels • Kinetic analysis • Lipid membranes • Sensor surfaces • SPR instruments |
| ISBN-10 | 1-60761-669-6 / 1607616696 |
| ISBN-13 | 978-1-60761-669-6 / 9781607616696 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
aus dem Bereich
