Calcium Signaling Protocols
Humana Press Inc. (Verlag)
978-0-89603-597-3 (ISBN)
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David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods - largely based on fluorescence measurement - to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin.
Part I. General. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations, Alec W. M. Simpson. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert. Measurement of [Ca2+]i in Cell Suspensions Using Indo-1, Adriaan Nelemans. Part II. Specialist Measurement Systems. Confocal Microscopy: Theory and Applications, Laura C. Mongan, Joanne Gormally, Andrew R. D. Hubbard, Christopher d'Lacey, and Colin D. Ockleford. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy, Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i), Anthony J. Morgan and Andrew P. Thomas. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR), Elaine Sullivan, Emily M. Tucker, and Ian L. Dale. Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity. Measurement of Ca2+ Entry Using 45Ca2+, Mercedes Villarroya, Manuela G. Lopez, Maria F. Cano-Abad, and Antonio G. Garcia. Measurement of [3H]PN200-110 and [125I]w-Contoxin MVIIA Binding, Kazuyoshi Hirota and David G. Lambert. Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores. Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells, Philip Swigart and Shamshad Cockcroft. Measurement of Inositol(1,4,5)triphosphate Using a Stereospecific Radioreceptor Mass Assay, Darren Smart. Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay, Robert A. Wilcox. Microinjection of myo-Inositol(1,4,5)triphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells, Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann. Photolysis of Caged Calcium Using a Low-Cost Flash Unit, Gommert A. van Koeveringe and Ron van Mastrigt. Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor - Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology), Edwin C. Thrower. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inosito(1,4,5)trisphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermeabilized Cells, Robert A. Wilcox and James Strupish. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators, Aldebaran M. Hofer. Part V. Specialist Measurement Techniques. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry, Hideo Kanaide. Measurement of Calcium and Movement in Heart Cells, Leong L. Ng and Paulene A. Quinn. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations, Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch. Measurement of Cytosolic-Free Ca2+ in Plant Tissue, Martin R. McAinsh and Irina Staxen. Part VI. Ca2+ Sensitive Targets. Assay and Purification of Calmodulin-Dependent Protein Kinase, Rakesh Kakkar and Rajendra K. Sharma. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1), Danuta Kosk-Kosicka. Index.
| Reihe/Serie | Methods in Microbiology Series ; No. 114 |
|---|---|
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 153 x 229 mm |
| Gewicht | 1259 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-89603-597-2 / 0896035972 |
| ISBN-13 | 978-0-89603-597-3 / 9780896035973 |
| Zustand | Neuware |
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