Agrobacterium: From Biology to Biotechnology (eBook)

Vitaly Citovsky, Tzvi Tzfira (Herausgeber)

eBook Download: PDF

2007 | 2008
XXXIV, 750 Seiten
Springer New York (Verlag)
978-0-387-72290-0 (ISBN)

Infos

Agrobacterium is a plant pathogen which causes the 'crown-gall' disease, a neoplastic growth that results from the transfer of a well-defined DNA segment ('transferred DNA', or 'T-DNA') from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications.

The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Dr. Tzvi Tzfira is an Assistant Professor in the Department of Molecular, Cellular, and Developmental Biology at the University of Michigan.

Dr. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at Stony Brook University.

---

Agrobacterium is a plant pathogen which causes the "e;crown-gall"e; disease, a neoplastic growth that results from the transfer of a well-defined DNA segment ("e;transferred DNA"e;, or "e;T-DNA"e;) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Inhalt

Contributing Authors 6
Table of Contents 11
Preface 27
Color Figures 29
Acknowledgments 30
AGROBACTERIUM: A DISEASE-CAUSING BACTERIUM 31
1 INTRODUCTION 32
2 AGROBACTERIUM HOST RANGE 34
3 DIVERSITY OF NATURAL ISOLATES 35
4 SOURCES OF INFECTION AND CONTROL OF CROWN GALL DISEASE 42
5 ACKNOWLEDGEMENTS 56
6 REFERENCES 56
A BRIEF HISTORY OF RESEARCH ON AGROBACTERIUM TUMEFACIENS: 1900-1980s 77
1 INTRODUCTION 77
2 AGROBACTERIUM— THE PATHOGEN 79
3 A. TUMEFACIENS AS THE VECTOR OF CHOICE FOR PLANT GENETIC ENGINEERING 89
4 CONCLUSIONS 94
5 ACKNOWLEDGEMENTS 94
6 REFERENCES 95
AGROBACTERIUM AND PLANT BIOTECHNOLOGY 103
1 INTRODUCTION 104
2 THE DEVELOPMENT OF AGROBACTERIUM-MEDIATED TRANSFORMATION 105
3 APPLICATIONS OF AGROBACTERIUM-MEDIATED TRANSFORMATION 121
4 GENE FLOW AND MOLECULAR APPROACHES TO TRANSGENE CONTAINMENT/ MONITORING 143
5 GLOBAL STATUS OF AGRICULTURAL BIOTECHNOLOGY AND TECHNOLOGY TRANSFER 146
6 ACKNOWLEDGEMENTS 152
7 REFERENCES 152
THE AGROBACTERIUM TUMEFACIENS C58 GENOME 178
1 INTRODUCTION 179
2 GENERAL FEATURES OF THE GENOME 179
3 THE LINEAR CHROMOSOME 181
4 PHYLOGENY AND WHOLE-GENOME COMPARISON 184
5 DNA REPLICATION AND THE CELL CYCLE 185
6 GENUS-SPECIFIC GENES 186
7 PLANT TRANSFORMATION AND TUMORIGENESIS 187
8 TRANSPORT 188
9 REGULATION 189
10 RESPONSE TO PLANT DEFENSES 191
11 GENERAL METABOLISM 192
12 CONCLUSIONS 195
13 ACKNOWLEDGEMENTS 198
14 REFERENCES 198
AGROBACTERIUM— TAXONOMY OF PLANT- PATHOGENIC RHIZOBIUM SPECIES 211
1 INTRODUCTION 212
2 HISTORICAL PERSPECTIVE— ORIGINS 213
3 AGROBACTERIUM—RHIZOBIUM RELATIONSHIPS 223
4 GENOTYPIC RELATIONSHIPS 224
5 PLASMID TRANSFER AND GENUS RECLASSIFICATION 227
6 DIVERSITY WITHIN RHIZOBIUM 228
7 REVISION OF AGROBACTERIUM NOMENCLATURE 232
8 RELATIONSHIP OF RHIZOBIUM TO OTHER MEMBERS OF THE RHIZOBIACEAE 235
9 OTHER ‘ AGROBACTERIUM’ SPECIES 236
10 SUMMARY 237
11 ACKNOWLEDGEMENTS 237
12 REFERENCES 238
THE INITIAL STEPS IN AGROBACTERIUM TUMEFACIENS PATHOGENESIS: CHEMICAL BIOLOGY OF HOST RECOGNITION 249
1 INTRODUCTION 250
2 SIGNAL DIVERSITY 251
3 SIGNAL RECOGNITION, INTEGRATION AND TRANSMISSION 254
4 SUMMARY 264
5 ACKNOWLEDGEMENTS 264
6 REFERENCES 264
AGROBACTERIUM-HOST ATTACHMENT AND BIOFILM FORMATION 270
1 INTRODUCTION 271
2 PRESUMPTIVE ADHERENCE FACTORS 274
3 PLANT RECEPTORS RECOGNIZED DURING A. TUMEFACIENS INFECTION 285
4 BIOFILM FORMATION BY A. TUMEFACIENS 286
5 A MODEL FOR ADHERENCE AND BIOFILM FORMATION 293
6 A WIDE RANGE OF SURFACE INTERACTIONS 294
7 CONCLUSIONS 295
8 ACKNOWLEDGEMENTS 296
9 REFERENCES 296
PRODUCTION OF A MOBILE T-DNA BY AGROBACTERIUM TUMEFACIENS 305
1 INTRODUCTION 306
2 A. TUMEFACIENS—NATURE’S GENETIC ENGINEER 306
3 INTERKINGDOM GENE TRANSFER 307
4 VirD2 INTERACTS WITH HOST PROTEINS 320
5 T-DNA INTEGRATION 323
6 PLANT GENETIC ENGINEERING 325
7 ACKNOWLEDGEMENTS 327
8 REFERENCES 327
TRANSLOCATION OF ONCOGENIC T-DNA AND EFFECTOR PROTEINS TO PLANT CELLS 340
1 INTRODUCTION 341
2 A HISTORICAL OVERVIEW 341
3 A. TUMEFACIENS VIRB/D4 SECRETION SUBSTRATES 345
4 THE VIRB/D4 MACHINE 351
5 VIRB/D4 MACHINE ASSEMBLY AND SPATIAL POSITIONING 358
6 VIRB/D4 CHANNEL/PILUS ARCHITECTURE 364
7 T-DNA TRANSLOCATION ACROSS THE CELL ENVELOPE 366
8 THE AGROBACTERIUM – PLANT CELL INTERFACE 372
9 SUMMARY AND PERSPECTIVES 375
10 ACKNOWLEDGEMENTS 377
11 REFERENCES 377
INTRACELLULAR TRANSPORT OF AGROBACTERIUM T- DNA 390
1 INTRODUCTION 390
2 STRUCTURE AND FUNCTION OF THE T-COMPLEX 392
3 CYTOPLASMIC TRANSPORT 397
4 NUCLEAR IMPORT 399
5 INTRANUCLEAR MOVEMENT OF THE T-COMPLEX 406
6 FROM THE CYTOPLASM TO THE CHROMATIN: A MODEL FOR T- COMPLEX IMPORT 407
7 FUTURE PROSPECTS 409
8 ACKNOWLEDGEMENTS 409
9 REFERENCES 410
MECHANISMS OF T-DNA INTEGRATION 420
1 INTRODUCTION 421
2 THE T-DNA MOLECULE 422
3 PROTEINS INVOLVED IN T-DNA INTEGRATION 423
4 GENOMIC ASPECTS OF T-DNA INTEGRATION/ TARGET- SITE SELECTION 433
5 MODELS FOR T-DNA INTEGRATION 445
6 FUTURE DIRECTIONS 453
7 ACKNOWLEDGEMENTS 454
8 REFERENCES 454
AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION: PATTERNS OF T- DNA INTEGRATION INTO THE HOST GENOME 466
1 INTRODUCTION 467
2 T-DNA INTEGRATION MECHANISM: SUCCESSIVE STEPS LEADING TO STABLE INTEGRATION OF THE T- DNA INTO THE PLANT HOST GENOME 468
3 PATTERNS OF T-DNA INTEGRATION INTO THE HOST GENOME 483
4 ACKNOWLEDGMENTS 494
5 REFERENCES 494
FUNCTION OF HOST PROTEINS IN THE AGROBACTERIUM- MEDIATED PLANT TRANSFORMATION PROCESS 507
1 INTRODUCTION 508
2 A GENETIC BASIS EXISTS FOR HOST SUSCEPTIBILITY TO AGROBACTERIUM-MEDIATED TRANSFORMATION 509
3 THE PLANT RESPONSE TO AGROBACTERIUM: STEPS IN THE TRANSFORMATION PROCESS, AND PLANT GENES/ PROTEINS INVOLVED IN EACH OF THESE STEPS 512
4 CONCLUSIONS 531
5 ACKNOWLEDGMENTS 532
6 REFERENCES 532
THE ONCOGENES OF AGROBACTERIUM TUMEFACIENS AND AGROBACTERIUM RHIZOGENES 547
1 INTRODUCTION 548
2 THE A. TUMEFACIENS ONCOGENES 549
3 THE A. RHIZOGENES ONCOGENES 557
4 CONCLUSIONS 573
5 REFERENCES 574
BIOLOGY OF CROWN GALL TUMORS 588
1 INTRODUCTION 589
2 CROWN GALL VASCULARIZATION 590
3 ONCOGENE-INDUCED PHYTOHORMONE CASCADE 593
4 ENHANCEMENT OF WATER AND SOLUTE TRANSPORT 602
5 KINETICS AND FUNCTION OF THE SUGAR-CLEAVING ENZYMES SUCROSE SYNTHASE, ACID CELL WALL AND VACUOLAR INVERTASE 607
6 CONCLUSIONS 607
7 ACKNOWLEDGEMENTS 608
8 REFERENCES 608
THE CELL-CELL COMMUNICATION SYSTEM OF AGROBACTERIUM TUMEFACIENS 615
1 INTRODUCTION 616
2 A MODEL OF QUORUM SENSING IN A. TUMEFACIENS 617
3 STRUCTURE AND FUNCTION STUDIES OF TRAR 625
4 TRAR IN TRANSCRIPTION ACTIVATION 630
5 QUORUM SENSING IN TUMOURS AND INFECTED PLANTS 635
6 ACKNOWLEGEMENTS 636
7 REFERENCES 637
HORIZONTAL GENE TRANSFER 645
1 INTRODUCTION 646
2 FOOTPRINT OF HORIZONTAL GENE TRANSFER FROM AGROBACTERIUM TO TOBACCO PLANTS 646
3 Other CT-DNAs 656
4 GENETIC TUMORS 657
5 ADVANTAGE OF CT-DNA AND CREATION OF NEW SPECIES 661
6 ACKNOWLEDGEMENTS 664
7 REFERENCES 664
AGROBACTERIUM-MEDIATED TRANSFORMATION OF NON- PLANT ORGANISMS 670
1 INTRODUCTION 671
2 NON-PLANT ORGANISMS TRANSFORMED BY AGROBACTERIUM 673
3 EXPERIMENTAL ASPECTS OF AGROBACTERIUM-MEDIATED TRANSFORMATION OF NON- PLANT ORGANISMS 677
4 ROLE OF VIRULENCE PROTEINS IN THE AGROBACTERIUM- MEDIATED TRANSFORMATION OF NON- PLANT ORGANISMS 679
5 TARGETED INTEGRATION OF T-DNA 680
6 PROTEIN TRANSFER FROM AGROBACTERIUM TO NON- PLANT HOSTS 685
7 PROSPECTS 685
8 ACKNOWLEDGEMENTS 687
9 REFERENCES 687
THE BIOETHICS AND BIOSAFETY OF GENE TRANSFER 697
1 INTRODUCTION 698
2 WHICH RISKS ARE RELEVANT? 705
3 CONCERNS BEYOND RISK ASSESSMENT 709
4 CONCLUSIONS 714
5 ACKNOWLEDGEMENTS 715
6 REFERENCES 715
AGROBACTERIUM-MEDIATED GENE TRANSFER: A LAWYER’S PERSPECTIVE 718
1 INTRODUCTION -WHY SHOULD A SCIENTIST CARE ABOUT A LAWYER’S VIEW OF AGROBACTERIUM? 719
2 SOME BASICS ABOUT PATENTS 728
3 AGROBACTERIUM-MEDIATED TRANSFORMATION AND PATENT LAW 732
4 CONCLUSIONS 751
5 ACKNOWLEDGEMENTS 752
6 REFERENCES 752
Index 755

Produkt-Details

• Erscheint lt. Verlag: 25.12.2007
• Zusatzinfo: XXXIV, 750 p.
• Verlagsort: New York
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Botanik
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• Themenwelt: Weitere Fachgebiete ► Land- / Forstwirtschaft / Fischerei
• Schlagworte: Agrobacterium • Biotechnology • crown gall disease • currentjks • DNA • Expression • Genetic Engineering • gene transfer • pathogen • Pathogene • plant genetic engineering • plant pathogens • Transport
• ISBN-10: 0-387-72290-4 / 0387722904
• ISBN-13: 978-0-387-72290-0 / 9780387722900