Live-Cell RNA Imaging

Methods and Protocols

Buch | Hardcover

220 Seiten

2024 | 2025 ed.
Springer-Verlag New York Inc.
978-1-0716-4247-4 (ISBN)

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This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.

Live-Cell Imaging of mRNA Using a Pepper RNA Tag.- A Method for Bioluminescence-Based RNA Monitoring Using Split-Luciferase Reconstitution Techniques.- A Catalytic Hairpin Assembly-Based Approach for RNA Imaging in Living Cells.- Live Single-Cell Transcriptional Dynamics in Plant Cells.- Live-Cell RNA Imaging with a DNA-Functionalized Metal-Organic Framework-Based Fluorescent Probe.- 3D Walking Nanomachine for Monitoring of miRNA in Living Cells.- Live-Cell Imaging of RNA G-Quadruplex with a Dual-Color Fluorescence Switch-On Probe.- Visualization of Endogenous RNA G-Quadruplex in Individual Genes in Single Cells.- Imaging and Quantifying Ribosomal Frameshifting Dynamics with Single-RNA Precision in Live Cells.- Benzo[c,d]indole-Quinoline-Based Deep-Red Emissive Probes for Live-Cell Imaging of Nucleolar RNA.- Directly Visualization of Hepatitis C Virus RNA in Living Cells and Mice.- Imaging of Viral Genomic RNA Replication with Nanoprobes.- A Near-Infrared Fluorescent Probe for Live-Cell Imaging of RNA in the Cancer Cells.- A Fluorogenic Sensor via Catalytic Hairpin Assembly for Precise Live-Cell Imaging of mRNA.- Live-Cell Imaging of miRNAs with the Combination of CHA and HCR Techniques.- Live-Cell Imaging of Multiple Endogenous mRNAs Using RNA Aptamers and Chemical Probes.

Erscheint lt. Verlag	6.2.2025
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	7 Illustrations, black and white; Approx. 220 p. 59 illus., 52 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Chemie ► Analytische Chemie
	Technik
ISBN-10	1-0716-4247-2 / 1071642472
ISBN-13	978-1-0716-4247-4 / 9781071642474
Zustand	Neuware