Food Allergens

Methods and Protocols

Buch | Hardcover

377 Seiten

2023 | 2024 ed.
Springer-Verlag New York Inc.
978-1-0716-3452-3 (ISBN)

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This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Food Allergens: Methods and Protocols serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components.

lt;p>1. Food Allergens of Plant and Animal Origin: Classification, Characteristics, and Properties

Beatriz Moya, Maria Carmen Dieguez, Jesus F. Crespo, and Beatriz Cabanillas

2. Purification of Food Allergens from Their Natural Sources: Chromatographic Methods

Sahil Gupta and Changqi Liu

3. Recombinant Production of Food Allergens in Yeast Pichia pastoris

Surapon Piboonpocanun

4. Yeast Surface Display Methodology for the Characterization of Food Allergens In Situ

Milica Popovic and Marija Gavrovic-Jankulovic

5. Characterization of Linear IgE-Binding Epitopes in Food Allergens

Changqi Liu, Sahil Gupta, and Jing Zhao

6. Characterization of T-Cell Epitopes in Food Allergens by Bioinformatic Tools

Shudong He and Fanlin Zhou

7. Phage Immunoprecipitation Sequencing (PhIP-Seq) for Analyzing Antibody Epitope Repertoires against Food Antigens

Ioanna Filimonova, Gabriel Innocenti, and Thomas Vogl

8. 1D-, 2D-Gel Electrophoresis, Immunoblotting, and Enzyme-Linked Immunosorbent Assay (ELISA) for the Study of Food Allergens

Joana Costa, Caterina Villa, and Isabel Mafra

9. Preparation of Blinded Food Matrixes for Clinical Oral Challenges

Julie A. Nordlee, Joe L. Baumert, and Steve L. Taylor

10. Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy

Ricarda Zeindl, Jana Unterhauser, Manuel Röck, Reiner Eidelpes, Sebastian Führer, and Martin Tollinger

11. Co-Culture of Human Dendritic and T Cells for the Study of Specific T Cell-Mediated Responses against Food Allergens

Mónica Martínez-Blanco, David Menchén-Martínez, Carmen Cámara, Rosina López-Fandiño, M. Cecilia Berin, and Daniel Lozano-Ojalvo

12. Evaluation of the Suppressive Capacity of Regulatory T Cells in Food Allergy Research

David Menchén-Martínez, Mónica Martínez-Blanco, Daniel Lozano-Ojalvo, and M. Cecilia Berin

13. Mass Cytometry in Food Allergy Research

Nathalie Acevedo and Kevin Llinás-Caballero

14. Indirect Basophil Activation Test for Peanut Allergy Diagnosis Using Human Donor Basophils

Janneke Ruinemans-Koerts and Yvonne Schmidt-Hieltjes

15. Standardization of Food Allergen Measurements Using Multiplex Array Technology

Kristina Reid Black, Stephanie C. Filep, Bryan R.E. Smith, and Martin D. Chapman

16. Biosensors for the Detection of Food Allergens

Alina Vasilescu, Cristina Polonschii, and Jean-Louis Marty

17. Standardization of a Mass Spectrometry-Based Workflow for Food Allergen Quantification

Rosa Pilolli, Elisabetta De Angelis, Antonella Lamonaca, and Linda Monaci

18. Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph

Catherine H. Schein

19. Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA)

Changqi Liu, Mengna Su, Valerie D. Zaffran, and Sahil Gupta

20. Detection of Bet v 1 Homologous Proteins and Plant Profilins by Indirect ELISA

Adrian Bartos, Iwona Majak, and Joanna Leszczynska

21. A Mouse Model of Shrimp Allergy with Cross-Reactivity to Crab and Lobster

Johanna M. Smeekens, Janelle R. Kesselring, Ken Bagley, and Michael D. Kulis

22. Animal Models in the Study of Food Allergens: Long-Term Maintenance of Allergic Reactivity in Mouse Models of Food Allergy

Jessica J. O'Konek

23. Study of MicroRNAs Expression in Food Allergy

Emilio Nuñez-Borque, Lucía Palacio-Garcia, Sergio Fernández-Bravo, and Vanesa Esteban

24. De Novo Transcriptomic Analyses to Identify and Compare Allergens in Foods

Shaymaviswanathan Karnaneedi, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, and Andreas L. Lopata

25. Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research

Fahd Alhamdan

Erscheinungsdatum	27.09.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	57 Illustrations, color; 16 Illustrations, black and white; XII, 377 p. 73 illus., 57 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Dermatologie
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Technik ► Lebensmitteltechnologie
Schlagworte	Allergenic components • Animal Models • Bioinformatics • Food allergy mechanisms • therapeutic targets
ISBN-10	1-0716-3452-6 / 1071634526
ISBN-13	978-1-0716-3452-3 / 9781071634523
Zustand	Neuware