Directed Evolution (eBook)

Methods and Protocols

Andrew Currin, Neil Swainston (Herausgeber)

eBook Download: EPUB

2022
Springer US (Verlag)
978-1-0716-2152-3 (ISBN)

Infos

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Produkt-Details

• Erscheint lt. Verlag: 21.6.2022
• Sprache: englisch
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• ISBN-10: 1-0716-2152-1 / 1071621521
• ISBN-13: 978-1-0716-2152-3 / 9781071621523